Sökning: "dot blot"

Visar resultat 6 - 10 av 12 avhandlingar innehållade orden dot blot.

1. 6. Genital human papillomaviruses. Studies of their occurrence, type spectrum and expression

   Författare :Ola Forslund; Malmö Klinisk mikrobiologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; mRNA expression; microwell; HPV 70; type determination; CIN; quantification; urine; multifocal infections; hybridization; PCR; HPV; human papillomavirus; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

   Sammanfattning : The occurrence of genital human papillomavirus infection was determined in different categories of patients by means of PCR. In high proportion of young women attending an adolescent clinic, the presence of HPV DNA was demonstrated in simultaneously urethral and cervical samples, suggesting that genital HPV infections are often multifocal. LÄS MER

3. 7. Affibody molecules for proteomic and therapeutic applications

   Författare :Caroline Grönwall; Stefan Ståhl; Lena Jendeberg; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody; protein engineering; phage display; amyloid beta peptide; transferrin; CD25; IL-2 receptor; proteomics; Bioengineering; Bioteknik;

   Sammanfattning : This thesis describes generation and characterization of Affibody molecules with future applications in proteomics research, protein structure determinations, therapeutic treatment of disease and medical imaging for in vivo diagnostics. Affibody molecules are engineered affinity proteins developed by combinatorial protein engineering from the 58-residue protein A-derived Z domain scaffold. LÄS MER

4. 8. Lipase-Lipid Interactions Studied by Site-Directed Labeling and Biological Spectroscopy

   Författare :Eva Hedin; Karl Hult; Marek Brzozowski; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; thermomyces lanuginous lipase; site-directed labeling; lipase-lipid interactions; Biochemistry; Biokemi;

   Sammanfattning : This thesis describes the study of lipase–lipid interactions of the triglyceride lipase Thermomyces lanuginosus lipase, TLL, by use of site-directed chemical labeling and biological spectroscopy. Several single-cysteine variants of TLL were produced, and labeled with probes containing either an affinity-, a fluorescence- or a spin-label functionality. LÄS MER

5. 9. Development of novel molecular methods for Campylobacter species and for Helicobacter pylori

   Författare :Kristina Blom; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Campylobacter; Helicobacter pylori; PCR; mRNA; hybridization; flow cytometry; HpaA; urease; NAP;

   Sammanfattning : Background and Aim: Different Campylobacter species and Helicobacter pylori are common causes of gastrointestinal infections that may result in diarrhea and chronic gastritis, respectively. The overall aim of this thesis was to develop novel methods for identification, differentiation and characterization of these bacteria. LÄS MER

7. 10. Use of affordable technology for the sensitive and specific diagnosis of onchocerciasis (river blindness)

   Författare :Gabriel Eduardo Guzmán Laparra; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Onchocerciasis or river blindness is the most common infectious cause of blindness. Even in the absence of blindness, the skin effects caused are of social stigma. It is the goal of the World Health Organization to eradicate filariasis (including onchocerciasis). LÄS MER