Sökning: "doktorsavhandling bioteknik kth"

Visar resultat 1 - 5 av 116 avhandlingar innehållade orden doktorsavhandling bioteknik kth.

  1. 1. Interaction engineered three-helix bundle domains for protein recovery and detection

    Författare :Tove Alm; Sophia Hober; Rainer Hahn; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ABD; Zbasic; albumin; protein engineering; phage display; staphylococcal display; inclusion bodies; refolding; proteomics; orthogonal affinity purification; Bioengineering; Bioteknik; Immunology engineering; Immunteknik;

    Sammanfattning : HTML clipboard The great advances in DNA technology, e.g. sequencing and recombinant DNA techniques, have given us the genetic information and the tools needed to effectively produce recombinant proteins. Recombinant proteins are valuable means in biotechnological applications and are also emerging as alternatives in therapeutic applications. LÄS MER

  3. 2. Single-particle cryo-electron microscopy of macromolecular assemblies

    Författare :Kimberley Cheng; Hans Hebert; Roland Beckmann; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioengineering; Bioteknik;

    Sammanfattning : In this thesis, single-particle cryo-electron microscopy (cryo-EM) was used to study the structure of three macromolecular assemblies: the two hemocyanin isoforms from Rapana thomasiana, the Pyrococcus furiosus chaperonin, and the ribosome from Escherichia coli. Hemocyanins are large respiratory proteins in arthropods and molluscs. LÄS MER

  4. 3. Towards unbiased 3D reconstruction : in single-particle cryo-electron microscopy

    Författare :Dominika Elmlund; Hans Hebert; Sarah Butcher; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioengineering; Bioteknik;

    Sammanfattning : Cryo-electron microscopy of freestanding molecules (single-particles) plays a pivotal role in the difficult and pressing challenge of determining the structures of large macromolecular complexes. Molecular volumes are generated by aligning large sets of randomly oriented two-dimensional (2D) projection images in three dimensions (3D) before reconstruction is performed using tomographic techniques. LÄS MER

  5. 4. On bacterial formats in protein library technology

    Författare :Per-Åke Löfdahl; Per-Åke Nygren; Mikael Widersten; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody molecule; β-lactamase; combinatorial library; green fluorescent protein GFP ; protein engineering; protein fragment complementation assay PCA ; selection; TEVprotease; TNF-α; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik;

    Sammanfattning : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. LÄS MER

  7. 5. Selection of antigens for antibody-based proteomics

    Författare :Lisa Berglund; Mathias Uhlén; Sören Brunak; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; epitope; antigen; antibody; affinity; protein; proteome; proteomics; bioinformatics; prediction; primer design; sequence similarity; Bioengineering; Bioteknik;

    Sammanfattning : The human genome is predicted to contain ~20,500 protein-coding genes. The encoded proteins are the key players in the body, but the functions and localizations of most proteins are still unknown. LÄS MER