Sökning: "dna primers"

Visar resultat 1 - 5 av 55 avhandlingar innehållade orden dna primers.

1. 1. Role of yeast DNA polymerase epsilon during DNA replication

   Författare :Isabelle Isoz; Erik Johansson; Juhani Syväoja; Umeå universitet; []
   Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; DNA polymerase epsilon; eukaryotic DNA replication; fidelity; leading strand; Dpb2; Biochemistry and clinical chemistry; Biokemi och klinisk kemi;

   Sammanfattning : Each cell division, the nuclear DNA must be replicated efficiently and with high accuracy to avoid mutations which can have an effect on cell function. There are three replicative DNA polymerases essential for the synthesis of DNA during replication in eukaryotic cells. LÄS MER

3. 2. Arrayed identification of DNA signatures

   Författare :Max Käller; Lundeberg Joakim; Mats Nilsson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; apyrase; allele-specific extension; competitive hybridization; DNA sequencing; genotyping; human papillomavirus HPV ; MC1R; microarray; mutation; p53; protease; Bioengineering; Bioteknik;

   Sammanfattning : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. LÄS MER

4. 3. Initiation of Mammalian Mitochondrial DNA Replication

   Författare :Elisabeth Jemt; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mitochondria; mtDNA; DNA replication;

   Sammanfattning : Mitochondria produce most of the adenosine triphosphate required in a eukaryotic cell and they contain their own genome. The mitochondrial DNA (mtDNA) is a double stranded circular molecule that codes for proteins required for cellular respiration and RNA molecules involved in translation of these proteins. LÄS MER

5. 4. A study of DNA interactive proteins and their origin from thermophilic Siphoviridae phages

   Författare :Josefin Ahlqvist; Bioteknik; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Thermophilic bacteriophages; Siphoviridae; Genome comparison; Protein production; DNA polymerase I; Holliday junction resolving enzyme; Structure–function relationship; Signature motifs; Bioinformatics;

   Sammanfattning : This thesis covers the results of studies on the amino acid sequence, three-dimensional structure, and activities of two types of DNA-interactive proteins: Holliday Junction resolving enzyme (represented by Hjc_15-6 isolated from phage Tth15-6) and DNA Polymerase I (represented by PolI_G20c, isolated from phage G20c), including studies on their homologous counterparts. As these proteins originate from two different, but related phages that infect Thermus thermophilus, the studies also include the evolutionary relationship between these and other related phages at genomic level. LÄS MER

7. 5. Diversity of thermostable DNA enzymes from Icelandic hot springs

   Författare :Sigridur Hjorleifsdottir; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; thermophiles; microbial diversity; SSU rRNA; Thermus; hot springs; Biotechnology; Bioteknik; Biochemistry; enzymologi; Proteiner; enzymology; metabolism; Proteins; DNA enzymes; Biokemi;

   Sammanfattning : The diversities of bacteria as well as thermostable DNA enzymes in Icelandic hot springs were studied using different techniques. First of all the bacterial diversity of different microbial mats as well as sediment samples was described by using both molecular methods and cultivation methods. LÄS MER