Sökning: "digitonin"

Hittade 5 avhandlingar innehållade ordet digitonin.

  1. 1. Techniques to manipulate the environment around and inside single cells

    Författare :Helen Bridle; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; digitonin; solution exchange; microfluidics; oscillations; temperature control; ion channels; valves;

    Sammanfattning : Several methods for extending the functionality of a commercially available microfluidic system have been developed to allow for the manipulation of the extracellular and intracellular environments. The microfluidic system generates a chemically patterned laminar flow in an open volume. LÄS MER

  3. 2. Biogenesis and assembly of the mammalian mitochondrial inner membrane

    Författare :Antek Wielburski; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : The aims of this investigation were to study the synthesis and assembly of mammalian mitochondrial inner membrane with emphasis on cytochrome c oxidase. Model systems utilizing rat hepatocytes and digitonin-treated hepatocytes were established for use in the investigation of mitochondrial protein synthesis in mammalian cells. LÄS MER

  4. 3. Light’EM up! : structural characterization of light-driven membrane protein complexes by cryogenic electron microscopy

    Författare :André Tomás Graça; Wolfgang P. Schröder; Johannes Messinger; Karina Persson; Werner Kühlbrandt; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; 3D reconstruction; Arabidopsis thaliana; assembly; chlorophyll; cryo-EM; cyanobacteria; Deinococus radiodurans; electron; land plants; membrane protein complexes; Photosystem II; proton; single-particle analysis; S-Layer Deinoxanthin Binding Complex SDBC ; water.; Biochemistry; biokemi; biologisk kemi; biological chemistry;

    Sammanfattning : Photosynthesis is probably the most important process for allowing life to develop into the diverse forms we see today. In this process, solar radiation is used to convert CO2 into biomass. From this process, we obtain oxygen to breathe, sources of food (plant biomass), and the potential for clean and sustainable energy. LÄS MER

  5. 4. A novel method using DNA binding fluorochromes as indirect probes to study linker histone-chromatin interactions in situ

    Författare :Helena Loborg; Pierre Åman; Linköpings universitet; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : Over the years, a variety of methods have been used to study chromatin structure. These techniques have provided valuable information about nuclear framework, chromatin organisation, and the proteins that are involved in the formation and maintenance of chromatin. LÄS MER

  7. 5. Fatty Acyl Reductases and Fatty Acyl Desaturases. Deciphering the biosynthesis of species-specific moth female sex pheromones from common fatty acids

    Författare :Åsa Hagström; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Moth; pheromone; insect; evolution; fatty acyl desaturase; fatty acyl reductase; endoplasmic reticulum; fatty acid biosynthesis; GFP; heterologous expression; phylogeny; cell factory; lepidoptera;

    Sammanfattning : Moths (Lepidoptera) are dependent on female produced pheromones, chemical signals, for attracting a mate. Pheromones are most commonly made up by saturated and/or unsaturated fatty acid derived alcohols, acetates, and/or aldehydes that are produced by a set of enzymes in the female pheromone gland. LÄS MER