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Visar resultat 1 - 5 av 99 avhandlingar som matchar ovanstående sökkriterier.

  1. 1. PCR inhibition mechanisms in forensic DNA analysis

    Författare :Maja Sidstedt; Teknisk mikrobiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PCR inhibition mechanisms; Real-time PCR; Digital PCR; Massively parallel sequencing;

    Sammanfattning : In forensic DNA analysis, the polymerase chain reaction (PCR) enables DNAanalysis of minute biological crime scene traces. PCR is an enzymatic reaction for amplification of specific DNA fragments involving both biochemical andbiophysical processes. The main analytical challenge is posed by the nature of the samples of interest. LÄS MER

  3. 2. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

    Författare :Amal Elfaitouri; Jonas Blomberg; Helene Norder; Uppsala universitet; []
    Nyckelord :Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

    Sammanfattning : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. LÄS MER

  4. 3. Dried blood sampling and digital readout to advance molecular diagnostics

    Författare :Johan Björkesten; Ulf Landegren; Björn Högberg; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; molecular diagnostics; dried blood sample; DBS; digital readout; digital enumeration; DNA detection methods; proximity extension assay; protein stability; genotyping; rare mutations; cell free DNA; multiplex protein measurement.; Farmaceutisk vetenskap; Pharmaceutical Science;

    Sammanfattning : A drastically increased capacity to measure large sets of molecular features in numerous patient samples in great detail will be required to fulfill the vision of precision medicine and wellness, which may characterize molecular diagnostics in the 21st century. Also sampling procedures need a renaissance to permit continuous sampling at population levels at reasonable cost. LÄS MER

  5. 4. Improved methodologies for molecular detection and quantification of viruses in food

    Författare :Sofia Persson; Patrik Ellström; Magnus Simonsson; Wim van der Poel; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; foodborne virus; digital PCR; real-time PCR; validation; assay design; food safety; Medicinsk vetenskap; Medical Science;

    Sammanfattning : Foodborne viruses such as norovirus, hepatitis A virus and hepatitis E virus cause a high burden of disease worldwide. Reverse transcription (RT) quantitative real-time PCR (qPCR) is the current standard method for monitoring viral contamination in the food chain. LÄS MER

  7. 5. Hepatitis B virus RNA in serum and liver tissue - quantification using digital PCR

    Författare :Kasthuri Prakash; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; hepatitis B virus; NTCP; HBV DNA; HBV RNA; subviral particles; integrations; droplet digital PCR;

    Sammanfattning : Hepatitis B virus (HBV) infection is a global health issue that is responsible for approximately 900,000 deaths each year, by inducing liver cirrhosis and hepatocellular carcinoma (HCC). A few markers are used to classify HBV infection and monitor treatment efficacy, including HBV DNA, surface antigen (HBsAg) and e antigen (HBeAg) in serum as well as HBV DNA and RNA in liver tissue. LÄS MER