Sökning: "digital enumeration"

Hittade 3 avhandlingar innehållade orden digital enumeration.

  1. 1. Dried blood sampling and digital readout to advance molecular diagnostics

    Författare :Johan Björkesten; Ulf Landegren; Björn Högberg; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; molecular diagnostics; dried blood sample; DBS; digital readout; digital enumeration; DNA detection methods; proximity extension assay; protein stability; genotyping; rare mutations; cell free DNA; multiplex protein measurement.; Farmaceutisk vetenskap; Pharmaceutical Science;

    Sammanfattning : A drastically increased capacity to measure large sets of molecular features in numerous patient samples in great detail will be required to fulfill the vision of precision medicine and wellness, which may characterize molecular diagnostics in the 21st century. Also sampling procedures need a renaissance to permit continuous sampling at population levels at reasonable cost. LÄS MER

  2. 2. Readout Strategies for Biomolecular Analyses

    Författare :Jenny Göransson; Mats Nilsson; Charles Cantor; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probe; Selector probe; Rolling circle amplification; Single molecule detection; Amplified single molecule; Random array; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ;

    Sammanfattning : This thesis describes three readout formats for molecular analyses. A common feature in all works is probing techniques that upon specific target recognition ideally results in equimolar amounts of DNA circles. These are then specifically amplified and detected using any of the techniques presented herein. LÄS MER

  3. 3. Single-Molecule Detection and Optical Scanning in Miniaturized Formats

    Författare :Jonas Melin; Fredrik Nikolajeff; Mats Nilsson; Hugh Fan; Uppsala universitet; []
    Nyckelord :Biotechnology; microfluidics; single-molecule detection; MOEMS; μTAS; lab-on-a-chip; RCA; thermoplastics; injection molding; hot embossing; Bioteknik;

    Sammanfattning : In later years polymer replication techniques have become a frequently employed fabrication method for microfluidic and micro-optical devices. This thesis describes applications and further developments of microstructures replicated in polymer materials. LÄS MER