Sökning: "depurination"

Hittade 3 avhandlingar innehållade ordet depurination.

  1. 1. Novel Methods for Synthesis of High Quality Oligonucleotides

    Författare :Andrey Semenyuk; Marek Kwiatkowski; Ulf Landegren; Joachim W. Engels; Uppsala universitet; []
    Nyckelord :Organic chemistry; oligonucleotide; solid-phase synthesis; RNA synthesis; phosphoramidite; abasic sites; depurination; 2 -O-DTM; protecting group; cartridge; conjugate; dithiomethyl linker; base-stable linker; oligonucleotide purification; nucleoside; Organisk kemi;

    Sammanfattning : The first part of the work describes a procedure of oligonucleotide purification using a reversed-phase cartridge. The developed method employs a very efficient yet mild oligonucleotide detritylation on the cartridge support allowing fast purification of oligonucleotides regardless of their 5´-modification. LÄS MER

  3. 2. Quantitative analysis of DNA adducts formed by some epoxides of industrial importance

    Författare :Kamila Plná; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :DNA adducts; 32P-postlabelling; cancer; genotoxicity; occupational; epoxides; allyl glycidyl ether; propylene oxide; epichlorohydrin;

    Sammanfattning : The work presented herein has focused on DNA adduct formation by three industrial epoxides, allyl glycidyl ether, propylene oxide and epichlorohydrin. Epoxides, owing to the strained oxirane ring are chemically reactive and can alkylate various sites in DNA. LÄS MER

  4. 3. DNA adducts formed by ethene and butadiene : quantitative analysis in rats and humans

    Författare :Chunyan Zhao; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :DNA adducts; 32P-postlabelling; cancer; genotoxicity; ethene; ethylene oxide; 1; 3-butadiene; epoxybutanediol;

    Sammanfattning : The present study has focused on DNA adduct formation by two important industrial and environmental chemicals, ethene and butadiene, in rats and humans. Ethene and butadiene are among the 50 chemicals produced in greatest volume in the United States. LÄS MER