Sökning: "dUTPases"

Hittade 4 avhandlingar innehållade ordet dUTPases.

  1. 1. Homotrimeric dUTPases : Principles of Catalysis and Inhibitor Design

    Författare :Lorena Gonzalez Palmén; Jan Kvassman; Dolores Gonzalez-Pacanowska; Högskolan i Kalmar; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; dUTPases; kinetics; ground state destabilization; inhibitors; Biochemistry; Biokemi; Natural Science; Naturvetenskap;

    Sammanfattning : The ubiquitous enzyme dUTPase hydrolyzes dUTP into dUMP and pyrophosphate, preventing DNA fragmentation and cell death due to accumulation of dUTP. Inhibitors of dUTPase could serve as drugs in the treatment of cancers and infectious diseases. This thesis presents five studies. LÄS MER

  3. 2. Viral dUTPases, Recombinant Expression, Purification and Characterization

    Författare :Anna-Carin Bergman; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; nucleocapsid protein; retrovirus; herpesvirus; dUTPase; nucleotide metabolism; enzymology; Proteiner; enzymologi;

    Sammanfattning : The enzyme deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase, EC 3.6.1.23) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate. LÄS MER

  4. 3. On Monomeric and Trimeric dUTPases. Recombinant Expression, Purification, Conformational Properties and Catalytic Characteristics

    Författare :Rebecca Persson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; dUTP; dUTPase; nucleotide metabolism; retrovirus; crystallography; herpesvirus; Biokemi; Biochemistry;

    Sammanfattning : Nucleotide metabolism and replication of nucleic acids are processes of fundamental importance. A crucial enzyme in nucleotide metabolism is dUTPase (E.C. 3. LÄS MER

  5. 4. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    Författare :Johan Nord; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Sammanfattning : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. LÄS MER