Sökning: "dATP?S"

Hittade 3 avhandlingar innehållade ordet dATP?S.

  1. 1. Pyrosequencing

    Författare :Samer Karamohamed; KTH; []
    Nyckelord :;

    Sammanfattning : Pyrosequencing is a method that can be used in almost allDNA sequencing applications. The method uses coupled reactionsof ATP sulfurylase and firefly luciferase to monitor theinorganic pyrophosphate (PPi) released during nucleotideincorporation onto a DNA primer. LÄS MER

  3. 2. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

    Författare :Jonas Eriksson; KTH; []
    Nyckelord :bioluminescence; osmolytes; glycine betaine; thermostability; firefly luciferase; inorganic pyrophosphatase; inorganic pyrophosphate; Pyrosequencing technology; secondary DNA-structures; Sequenase; Klenow-polymerase; reaction rates; temperature;

    Sammanfattning : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. LÄS MER

  4. 3. Method development and applications of Pyrosequencing technology

    Författare :Baback Gharizadeh; KTH; []
    Nyckelord :apyrase; bacterial identification; dATP?S; EST sequencing; fungal identification; human papillomavirus HPV ; microbial and viral typing; multiple sequencing primer method; Pyrosequencing technology; Sequenase; single-stranded DNA-binding protein SS;

    Sammanfattning : The ability to determine nucleic acid sequences is one ofthe most important platforms for the detailed study ofbiological systems. Pyrosequencing technology is a relativelynovel DNA sequencing technique with multifaceted uniquecharacteristics, adjustable to different strategies, formatsand instrumentations. LÄS MER