Sökning: "cyclic cystine knot"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden cyclic cystine knot.

  1. 1. Engineering of the Ultra-stable Cystine Knot Framework of Microproteins : Design, Chemical Synthesis and Structural Studies

    Författare :Teshome Leta Aboye; Ulf Göransson; Julio A. Camarero; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cyclotides; cystine knot; protein engineering; NMR; solid phase; disulfide rich; ultra-stable; microprotein; PHARMACY; FARMACI; Medicinal Chemistry; Läkemedelskemi;

    Sammanfattning : Ultra-stable cystine knotted microproteins, in which two disulfides and their connecting backbones form a circle that is penetrated by the third disulfide bonds, have attracted high interest due to their resistance to degradation in vitro and potential for the development of peptide drugs. This thesis gives new insights into engineering of that framework of microproteins, including approaches to their chemical synthesis, backbone engineering, structural and biological evaluations. LÄS MER

  3. 2. Studies on cysteine-rich peptides from Nemertea and Violaceae : Proteomic and transcriptomic discovery and characterization

    Författare :Erik Jacobsson; Ulf Göransson; Håkan S. Andersson; Robert Burman; Espen Hansen; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Peptide toxin; cystine knot; nemertide; cyclotide; nemertea.; Farmakognosi; Pharmacognosy;

    Sammanfattning : The overall aims of the projects included in this thesis were to discover, synthesize and characterize disulphide-stabilized peptides from marine worms (Nemertea sp.) and plants (Viola sp.). LÄS MER

  4. 3. Cyclotides evolve : Studies on their natural distribution, structural diversity, and activity

    Författare :Sungkyu Park; Göransson Ulf; Lars Bohlin; Mohamed Marahiel; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; cyclotide; cyclic cystine knot; evolution; peptide synthesis; chemical ligation; QSAR; Viola; Violaceae; phylogeny; Farmakognosi; Pharmacognosy;

    Sammanfattning : The cyclotides are a family of naturally occurring peptides characterized by cyclic cystine knot (CCK) structural motif, which comprises a cyclic head-to-tail backbone featuring six conserved cysteine residues that form three disulfide bonds. This unique structural motif makes cyclotides exceptionally resistant to chemical, thermal and enzymatic degradation. LÄS MER

  5. 4. Structure and Activity of Circular Plant Proteins : Cytotoxic Effects of Viola Cyclotides

    Författare :Anders Herrmann; Ulf Göransson; Lars Bohlin; Douglas Kinghorn; Uppsala universitet; []
    Nyckelord :Pharmacognosy; cyclotide; cytotoxic; anti-tumour; macrocyclic; cyclic cystine knot; Alpine violet; Sweet violet; Viola; Violaceae; chemical modifications; structure-activity studies; mass spectroscopy; cDNA clones; Farmakognosi;

    Sammanfattning : Cyclotides are a family of small and macrocyclic proteins that have been found in Violacaee and Rubiaceae plant species. These proteins contain a cystine knot: two disulfides bonds together with their connecting peptide backbone form an embedded ring which is penetrated by a third disulfide bond. LÄS MER

  7. 5. Cytotoxic Cyclotides : Structure, Activity, and Mode of Action

    Författare :Erika Svangård; Per Claeson; Lars Bohlin; Ulf Göransson; John H. Cardellina II; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmacognosy; cyclotide; cytotoxic; cancer; cyclic cystine knot; Viola; Violaceae; liposome; hollow fibre; homology modelling; mass spectrometry; amphipathic; structure–activity; tumour; membrane disruption; necrosis; Farmakognosi; Pharmacognosy; Farmakognosi;

    Sammanfattning : Cyclotides are small cyclic plant proteins, and this thesis addresses their cytotoxic structure-activity properties and their mode of action on human cancer cell lines. Cyclotides were isolated from Viola odorata and Viola tricolor; three novel cyclotide sequences and two known sequences, but of new origin, were identified using mass spectrometry, amino acid analysis, and Edman degradation. LÄS MER