Sökning: "cryo-EM"

Visar resultat 6 - 10 av 43 avhandlingar innehållade ordet cryo-EM.

  1. 6. Elucidating the molecular basis of Na+/H+ exchange

    Författare :Pascal F. Meier; David Drew; Simon Newstead; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; membrane protein; secondary-active transporter; Solute Carrier Transporter; ion-exchange; sodium proton exchanger; potassium proton exchanger; protein structure; cryo-EM; regulation; biokemi; Biochemistry;

    Sammanfattning : Solute carrier (SLC) transporters are membrane transport proteins, which catalyse the movement of nutrients, ions, and drugs across cell membranes. Here, I will present our contribution to understanding the mechanism of the sodium/proton exchangers (NHE), belonging to the SLC9 family of membrane transporters. LÄS MER

  3. 7. Evolutionary Mechanisms Shaping Bacterial Translation Termination

    Författare :Shreya Pundir; Suparna Sanyal; Martin Ott; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Ribosome; Protein; Release Factor; Translation; Termination; Stop codon; Evolution; Mutation; Kinetics; Cryo-EM; Molekylär biovetenskap; Molecular Life Sciences;

    Sammanfattning : Translation termination in bacteria involves precise reading of stop codons (UAA, UAG, UGA) and coordinated peptidyl-tRNA hydrolysis by the class-I release factors (RFs) on the ribosome (70S). This thesis investigates the evolutionary and post-translational modification mechanisms of these RFs and the concurrent effect on bacterial translation termination. LÄS MER

  4. 8. Peering Beyond the Noise in Experimental Biophysical Data

    Författare :Dari Kimanius; Erik Lindahl; Holger Stark; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cryo-EM; mitochondrial ribosome; SAXS; molecular dynamics; metadynamics; Biochemistry towards Bioinformatics; biokemi med inriktning mot bioinformatik;

    Sammanfattning : Experimental protein structure determination methods make up a fundamental part of our understanding of biological systems. Manual interpretation of the output from these methods has been made obsolete by the sheer size and complexity of the acquired data. LÄS MER

  5. 9. Structural Details of Human Aquaporin Regulation : Three stories of three aquaporins

    Författare :Veronika Nesverova; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aquaporin; membrane proteins; protein trafficking; water channel; aquaporin 0; aquaporin 5; aquaporin 2; cryo-EM; protein phosphorylation; protein-protein interaction; aquaporin regulation; aquaporins;

    Sammanfattning : Every cell is surrounded by a thin plasma membrane, protecting it from its surrounding. Specialized protein channels and transporters are embedded in this membrane, ensuring selective transport of molecules in and out of the cells. To maintain optimal hydration, water channels called aquaporins (AQPs) are present in every cell of our body. LÄS MER

  7. 10. Reverse structural biology: Cellular processes uncovered by electron microscopy

    Författare :Jacob Croft; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; flagella; nuclear envelope budding; TAILS; cryo-EM; microtubule inner protein;

    Sammanfattning : This thesis contains two projects, both using electron microscopy as the primary technique. In the first project, nuclear envelope budding (NEB) is investigated as an alternate method of transport across the nuclear envelope. LÄS MER