Sökning: "coupled folding"

Visar resultat 6 - 10 av 22 avhandlingar innehållade orden coupled folding.

1. 6. Zinc in folding and misfolding of SOD1 : Implications for ALS

   Författare :Lina Leinartaité; Mikael Oliveberg; Bengt-Harald Jonsson; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein stability; protein misfolding; local unfolding; metal binding; energy landscape; protein disease; amyotrophic lateral sclerosis; Biochemistry; biokemi;

   Sammanfattning : Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease causing degeneration of upper and lower motor neurons. Most ALS cases are sporadic; only 6% are associated with mutations in Cu, Zn superoxide dismutase (SOD1). LÄS MER

3. 7. Membrane chaperones : protein folding in the ER membrane

   Författare :Jhansi Kota; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : The plasma membrane (PM), comprised largely of lipids and proteins, is a dynamic structure that establishes the integrity of cells. Newly synthesized PM proteins are initially inserted into the endoplasmic reticulum (ER) prior to being targeted to the PM via the secretory pathway. Many PM proteins are polytopic, i.e. LÄS MER

4. 8. Folding and interaction studies of subunits in protein complexes

   Författare :Ximena Aguilar; Stefan Björklund; Alexis Verger; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Macromolecular crowding; cpn10; Mediator; Med25; Dreb2a; VP16; conformational changes; NMR; ITC.; biokemi; Biochemistry; molekylärbiologi; Molecular Biology;

   Sammanfattning : Proteins function as worker molecules in the cell and their natural environment is crowded. How they fold in a cell-like environment and how they recognize their interacting partners in such conditions, are questions that underlie the work of this thesis. LÄS MER

5. 9. Systems biology of protein synthesis and secretion in yeast

   Författare :Qi Qi; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; protein synthesis; multi-omics analysis; protein secretion; genome-scale modeling;

   Sammanfattning : Protein synthesis and secretion is a vital process to maintain cell function. As it demands numerous building blocks, cofactors and chaperones generated from metabolism and translation, the process is intertwined with metabolic and regulatory networks. LÄS MER

7. 10. Metabolic Engineering of Recombinant Protein Production by Saccharomyces cerevisiae

   Författare :Zihe Liu; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; genome sequencing; DNA microarray; insulin precursor; growth rate; UV mutation; yeast; anaerobic electron acceptor; secretory pathway; unfolded protein response; metabolic engineering; systems biology; α-amylase;

   Sammanfattning : The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and it also provides a platform for the production of many heterologous proteins of medical or industrial interest. In this thesis, random and rational approaches, such as vector design, host engineering, fermentation analysis, UV Mutation, coupled with high-throughput systems biology techniques (including whole genomic sequencing, microarray analysis and flux analysis) and integrated analysis (Reporter feature technique), were employed to engineer cellular properties more effectively and purposefully to construct cell factories for protein production. LÄS MER