Sökning: "conformational changes"

Visar resultat 6 - 10 av 196 avhandlingar innehållade orden conformational changes.

  1. 6. Improving selectivity in protein extraction by subtle interactions and environmental changes

    Författare :Sheryl Fernandes; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Difference Spectroscopy; Circular dichroism; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi; Fluorescence spectroscopy; Metal ions; Cibacron blue; Polyvinylpyrrolidone; Polyethylene glycol; Triton X-114; His 6 tag; Cold-active ß-lactamase; Cold-active ß-galactosidase; Lactate dehydrogenase; Cutinase; Affinity Extraction; Conformational change; Partial Denaturation; Molecular interaction; Aqueous two-phase systems; Detergent foam;

    Sammanfattning : The challenges for efficient bioprocesses lie in developing and adapting further current technologies or in creating innovative separation technologies. The thesis is based on the studies on protein recovery from crude feedstocks by extraction in aqueous two-phase systems and detergent foam, respectively. LÄS MER

  3. 7. Molecular Recognition and Conformational Dynamics in Macromolecules

    Författare :Soumendranath Bhakat; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein; ligand; host-guet; funnel metadynamics; MM PBSA; well-tempered metadynamics; collective variable; tyrosine; aspartic protease; local fluctuations; hydrogen exchange; time-lagged independent component analysis; principal component analysis; parallel-tempering;

    Sammanfattning : Computational methods gained a widespread use in drug discovery. Understanding conformational dynamics of protein and mechanisms of protein-ligand binding are two major areas in drug discovery. Molecular dynamics (MD) simulation have been routinely used to study conformational dynamics of protein and mechanisms of protein-ligand binding. LÄS MER

  4. 8. From Static Structures to Free Energy Landscapes: Characterizing Conformational Transitions in Biological Macromolecules

    Författare :Cathrine Bergh; Erik Lindahl; Gerhard Hummer; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein conformational transitions; molecular dynamics simulations; statistical mechanics; ion channels; Proteinkonformationsövergångar; molekyldynamiksimuleringar; statistisk mekanik; jonkanaler;

    Sammanfattning : To be alive means to temporarily counteract the fundamental dispersive driving force described by the second law of thermodynamics, eventually leading all systems to decay and disorder. In cells, this task is partly carried out by proteins - small specialized molecular machines that utilize free energy to maintain the proper functioning of the cell. LÄS MER

  5. 9. Conformational changes in Staphylococcus aureus' alpha-toxin : from water-soluble monomer to a transmembrane channel

    Författare :Beatrix Vécsey Semjén; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Staphylococcal alpha-toxin membrane-protein interaction pore formingtoxins.;

    Sammanfattning : Alpha-toxin is secreted by Staphylococcus aureus as a soluble monomer that without further activation can oligomerize and form a transmembrane channel. One of the aims of the present work was to understand the underlying mechanisms of the membrane insertion process. LÄS MER

  7. 10. Conformation of polypeptides at nanoparticles interfaces : protein structural changes and induced folding of peptides

    Författare :Martin Lundqvist; Fredrik Höök; Linköpings universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : This thesis can be divided into two parts. The first deals with characterization of structural and dynamic consequences for proteins of interactions with a solid surface in the form of silica nanoparticles. LÄS MER