Sökning: "condensation of DNA"

Visar resultat 1 - 5 av 34 avhandlingar innehållade orden condensation of DNA.

1. 1. Association of Amphiphiles and Polyelectrolytes with DNA

   Författare :Yulia S Mel'nikova; Fysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physical chemistry; cryo-TEM; SAXS; fluorescence microscopy; DNA condensation; DNA-polyelectrolyte; DNA- amphiphile interactions; Fysikalisk kemi;

   Sammanfattning : DNA- cationic amphiphile and DNA-cationic polymer systems were studied with respect to the intermolecular interactions, as well as structure and stability of formed complexes. The thesis is divided in two parts: one section is a summary and the other section contains five papers. LÄS MER

3. 2. Controlling DNA compaction and the interaction with model biomembranes

   Författare :Marie-Louise Ainalem; Fysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; model biomembrane; DNA compaction; cryo-TEM; null ellipsometry; dynamic light scattering; toroid; bilayer; neutron reflectivity; dendrimer; PAMAM; DNA; poly amido amine ;

   Sammanfattning : The studies described in this thesis forms part of a larger collaborative project, Neonuclei, with the objective to design a module for packaging the negatively charged DNA into a ”neonucleus” with transcription competence. There are many applications that require control of DNA compaction, i.e. packaging or condensation. LÄS MER

4. 3. Condensation of DNA for Gene Delivery: Studies on Vector-Nucleic Acid Interactions and Transfection Efficiency

   Författare :Kristina Fant; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; dendrimer; endocytosis; uptake; gene delivery; optical spectroscopy; structure-activity relationship; DNA; cell-penetrating peptide;

   Sammanfattning : This thesis concerns the delivery of nucleic acids into cells and tissues with the aim to change or correct gene expression. The field of gene delivery has the ultimate goal of producing genetic drugs for clinical applications, and the activity in this area is intense although so far the use is limited to research purposes. LÄS MER

5. 4. Charged Polymers: From DNA to Polyampholytes

   Författare :Malek O Khan; Beräkningskemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; computational steering; DNA condensation; ion correlations; electrostatic interactions; colloidal stability; polyampholytes; Monte Carlo simulations; polyelectrolytes; Theoretical chemistry; quantum chemistry; Teoretisk kemi; kvantkemi;

   Sammanfattning : Three main themes are outlined in this thesis: Polyelectrolytes/DNA : In a living cell, DNA molecules have a compact conformation in order to fit in, while in a test tube they will take on an expanded cylindrical shape, due to the negative phosphate groups along the DNA molecule. With the help of computer simulations it has been shown that like-charged monomers of a poly- electrolyte can attract each other if the counterions are taken into account. LÄS MER

7. 5. Physics of Viral Infectivity: Energetics of Genome Ejection

   Författare :Meerim Jeembaeva; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; microfluidics.; differential scanning calorimetry; capsid; Key words: phage; osmotic suppression; DNA ejection; isothermal titration calorimetry; enthalpy;

   Sammanfattning : All viruses that infect bacteria, plant, or animal cells involve a genome (RNA or DNA) that is encapsidated by a rigid protein shell. After delivery of the viral genome into the host cell, new capsid proteins, which are encoded by viral DNA or RNA, are expressed and self-assembled into new viral capsids. LÄS MER