Sökning: "clinical chemistry sample preparation"

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden clinical chemistry sample preparation.

1. 1. Immuno-affinity interactions in sample preparation and analysis

   Författare :Madalina Tudorache; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; immuno supported liquid membrane ISLM extraction; sample preparation; immunoassay; immuno-affinity interaction; antibody; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. LÄS MER

3. 2. Advances in analytical methodologies for studies of the platinum metallome in malignant cells exposed to cisplatin

   Författare :Yvonne Nygren; Erik Björn; Knut Irgum; Bente Gammelgaard; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Method development; Metallome; Inductively coupled plasma mass spectrometry; Electrospray ionization mass spectrometry; Liquid chromatography; Hyphenation; Organic modifier; Cisplatin; Platinum; Analytical chemistry; Analytisk kemi; analytisk kemi; Analytical Chemistry;

   Sammanfattning : The scientific progress about the important chemotherapeutic drug substance cisplatin (CDDP) and its function has often been rendered by data difficult to interpret, and still many questions about its mode of action remains to be clarified by the scientific community. However, studies of CDDP possess a high complexity due to; i) low intracellular concentration, ii) many potential biomolecule targets, iii) poor or unknown stability of the intact drug and its biomolecule adducts and iv) complex and varying sample matrices. LÄS MER

4. 3. Development of Liquid-based Separation Techniques using Tailored Surfaces for Analysis of Biological Samples

   Författare :Emilia Hardenborg; Jonas Bergquist; Marit Andersson; Alejandro Cifuentes; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aqueous humor; biological samples; capillary electrophoresis; coatings; drugs; liquid chromatography; molecular imprinted polymers; peptides; proteins; pseudoexfoliation; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Development and improvement of analytical techniques are vital in analytical chemistry research. This thesis describes the development and use of tailored surfaces for bioanalytical applications. LÄS MER

5. 4. Surface Modified Capillaries in Capillary Electrophoresis Coupled to Mass Spectrometry : Method Development and Exploration of the Potential of Capillary Electrophoresis as a Proteomic Tool

   Författare :Aida Zuberovic; Jonas Bergquist; Ziad El Rassi; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Topology optimization; Design optimization; Material distribution; Wave propagation problems; Inverse problems; Acoustic devices; Medical microwave tomography; High performance computing; Analytical chemistry; Analytisk kemi;

   Sammanfattning : The increased knowledge about the complexity of the physiological processes increases the demand on the analytical techniques employed to explore them. A comprehensive analysis of the entire sample content is today the most common approach to investigate the molecular interplay behind a physiological deviation. LÄS MER

7. 5. Mass Spectrometric Applications for Diagnosing Metabolic and Endocrine Diseases

   Författare :Mark M. Kushnir; Jonas Bergquist; Alan L Rockwood; David Herold; Uppsala universitet; []
   Nyckelord :Analytical chemistry; biomarker; liquid chromatography; tandem mass spectrometry; LC-MS MS; sample preparation; isomers; steroid hormones; congenital adrenal hyperplasia; polycystic ovary syndrome; Analytisk kemi;

   Sammanfattning : Disease-specific compounds (biomarkers) are analyzed in clinical laboratories to assist with diagnosing diseases. This thesis describes development and validation of liquid chromatography tandem mass spectrometry (LC-MS/MS) based tests for diagnosing a diverse group of endocrine and metabolic diseases. LÄS MER