Sökning: "choline oxidase"

Hittade 3 avhandlingar innehållade orden choline oxidase.

  1. 1. Creating Ultrafast Biosensors for Neuroscience

    Författare :Yuanmo Wang; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; quantification; glucose; choline oxidase; gold nanoparticles; enzyme monolayer; enzymatic activity; Biosensor; adsorption; brain slice; enzyme stability; acetylcholine esterase; artificial cell; exocytosis; glucose oxidase; deformation; glutamate; glutamate oxidase; acetylcholine; amperometry;

    Sammanfattning : Neuronal communication is the basis for all our brain function and relies on regulated exocytosis, a cell function that involves release of quantal amounts of neurotransmitters into the gap space between interconnected neurons to serve as chemical signals. To study exocytosis, which is a fast process that occurs on the timescale of sub-milliseconds to milliseconds, a toolbox of analytical methods has been developed where the electrochemical based techniques offer quantitative and sufficient high temporal recording speed. LÄS MER

  3. 2. Development of Ultra-fast Biosensors for Detection of Non-electroactive Neurotransmitters

    Författare :Yuanmo Wang; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; artificial cell; quantification; stability; deformation; dynamic light scattering; adsorption; amperometry; nanoparticle tracking analysis; enzyme monolayer; glucose oxidase; exocytosis; choline oxidase; acetylcholine; enzymatic activity; acetylcholine esterase; gold nanoparticles; Biosensor;

    Sammanfattning : During neuronal communication neurotransmitters are released through the process exocytosis that occurs on the time scale of sub-milliseconds to milliseconds. Neuronal activity by neurotransmitters that are non-electroactive cannot easily be monitored due to limitations in the temporal resolutions of the sensor probes for these analytes. LÄS MER

  4. 3. New Electrochemical Tools to Study Exocytosis

    Författare :Joakim Wigström; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; amperometry; QCM-D; micro electrode array; large dense core vesicles; chromaffin cells; PC-12 cells; microelectrode; exocytosis; electrochemical cytometry; electrochemical imaging; acetylcholine; endocytosis; nanoparticles; enzyme biosensor; microfabrication;

    Sammanfattning : The work described in this thesis has the focus on the development of new analytical tools to study processes related to cellular secretion (exocytosis) in cell models. Four novel techniques were developed, allowing new ways to study processes related to exocytosis, and gain previously unattainable knowledge. LÄS MER