Sökning: "chimeric"

Visar resultat 1 - 5 av 153 avhandlingar innehållade ordet chimeric.

  1. 1. Chimeric gene delivery vectors : Design, synthesis, and mechanisms from transcriptomics analysis

    Författare :Moataz Dowaidar; Ülo Langel; Sandrine Sagan; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell-penetrating peptides; magnetic nanoparticles; graphene oxide; autophagy; siRNA; SCO; QSAR; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

    Sammanfattning : Delivery of nucleic acid is a promising approach for genetic diseases/disorders. However, gene therapy using oligonucleotides (ONs) suffers from low transfection efficacy due to negative charges, weak cellular permeability, and enzymatic degradation. LÄS MER

  3. 2. Sorting and processing of neutrophil granule proteins

    Författare :Elinor Bülow; Avdelningen för hematologi och transfusionsmedicin; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; secretion; Haematology; extracellular fluids; Hematologi; extracellulära vätskor; cell-specific retention; chimeric; lysosome-related organelles; secretory pathway; propeptide of MPO; azurophil granules;

    Sammanfattning : The neutrophil granulocytes have a critical role in innate immunity in particular through phagocytosis of microorganisms. Their cytoplasmic granule subsets store digestive enzymes and antibiotic proteins that are released during this event. LÄS MER

  4. 3. Engineering of Antibacterial Phage-Derived Proteins

    Författare :Fredrik Seijsing; Anders Nilsson; Yves Briers; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein engineering; endolysin; IgG; linker protein; chimeric protein; artificial opsonin; spider silk; antibacterial surface; bacteriophage; structure prediction; molekylär biovetenskap; Molecular Bioscience;

    Sammanfattning : The increasing threat of antibiotic resistance calls for the development of new treatment methods. Bacteriophages are interesting candidates since they can lyse bacteria with great efficiency. Bacteriophages produce enzymes called endolysins which break down the peptidoglycan in the cell wall at the end of the infection cycle. LÄS MER

  5. 4. Characterization of galanin receptors using chimeric peptides and site-directed mutagenesis

    Författare :Ulla Eriksson Sollenberg; Ülo Langel; Anna-Lena Hulting; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; galanin; GPCR; mutagenesis; neuropeptide; Neurochemistry; Neurokemi; Neurochemistry and Neurotoxicology; neurokemi och neurotoxikologi;

    Sammanfattning : Galanin, a 29 (30) amino acid neuropeptide, is found throughout both the central and peripheral nervous systems. It signals via three receptors, GalR1-3, all belonging to the rhodopsin-like G-protein coupled receptors. Galanin and its receptors have been implicated in a vast variety of biological processes. LÄS MER

  7. 5. Mutational Analysis and Redesign of Alpha-class Glutathione Transferases for Enhanced Azathioprine Activity

    Författare :Olof Modén; Bengt Mannervik; Mikael Widersten; Kenneth Tew; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; allelic variants; azathioprine; bioactivation; chimeric mutagenesis; directed evolution; DNA shuffling; enzyme engineering; glutathione transferase; GST; lysate screening; molecular docking; multiple alignment; multivariate analysis; polymorphism; principal component analysis; prodrug; prodrug activation; protein engineering; protein redesign; reduced amino acid alphabet; saturation mutagenesis; semi-rational enzyme engineering; site-directed mutagenesis; structure-activity relationship; structure-based redesign; Biokemi; Biochemistry;

    Sammanfattning : Glutathione transferase (GST) A2-2 is the human enzyme most efficient in catalyzing azathioprine activation. Structure-function relationships were sought explaining the higher catalytic efficiency compared to other alpha class GSTs. LÄS MER