Sökning: "cell-free protein synthesis"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden cell-free protein synthesis.

  1. 1. Versatile Implementations of an Improved Cell-Free System for Protein Biosynthesis Functional and structural studies of ribosomal protein L11 and class II release factor RF3. Novel biotechnological approach for continuous protein biosynthesis

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Lamine Bouakaz; Uppsala universitet.; [2006]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Protein synthesis; translation termination; ribosome; release factor; cell-free system; missense error; gel filtration; affinity chromatography; Molekylärbiologi; NATURAL SCIENCES Biology Cell and molecular biology Molecular biology; NATURVETENSKAP Biologi Cell- och molekylärbiologi Molekylärbiologi;

    Sammanfattning : Advances in genetics, proteomics and chromatography techniques have enabled the successfully generation of a cell-free bacterial translation system composed of highly pure and active components. This system provided an ideal platform for better elucidating the mechanism of each individual step of the prokaryotic protein biosynthesis and the function of the translation factors involved in the process. LÄS MER

  2. 2. The Protein Traffic on the Ribosome The Mechanism and Regulation of Protein Synthesis in Prokaryotes

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Andrey Zavialov; Uppsala universitet.; [2004]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; yellow; ribosome; translation; Gagarin; nobel prize; Biokemi; NATURAL SCIENCES Chemistry Biochemistry; NATURVETENSKAP Kemi Biokemi;

    Sammanfattning : The aim of this work was to understand the molecular mechanism of translation and the mechanism of translation termination, in particular. Cleavage of peptidyl-tRNA and peptide release terminates translation of mRNA on the ribosome. In prokaryotes, three release factors (RFs) are involved in this process. LÄS MER

  3. 3. Insight into the mitochondrial apoptotic pathway The interplay of the pro-apoptotic Bax protein with oxidized phospholipids and its counterplayer, the pro-survival Bcl-2 protein

    Detta är en avhandling från Umeå : Umeå universitet

    Författare :Marcus Wallgren; Umeå universitet.; [2012]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; apoptosis; Bax; Bcl-2; CD; cell-free protein synthesis; DSC; membrane; mitochondria; oxidized phospholipids; reconstitution; solid state NMR; biologisk kemi; biological chemistry;

    Sammanfattning : Apoptosis plays a crucial role in multicellular organisms by preserving tissue homeostasis and removing harmful cells. The anti-apoptotic B-cell CLL/lymphoma 2 (Bcl-2) and the pro-apoptotic Bcl-2-associated X protein (Bax) act as major regulators of the mitochondrial apoptotic pathway. LÄS MER

  4. 4. Intrinsically Disordered Domains of the B Cell Receptor Cell-Free Expression and Characterization by NMR

    Detta är en avhandling från Umeå : Umeå universitet

    Författare :Linnéa Isaksson; Göteborgs universitet.; Gothenburg University.; [2014]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Naturvetenskap;

    Sammanfattning : After the last twenty years of research, the occurrence of flexible proteins without a fixed three-dimensional structure are no longer considered to be rare exceptions from the structure-function paradigm. Instead, intrinsically disordered proteins (IDPs) have become one of the most interesting subjects of modern protein research. LÄS MER

  5. 5. Mechanisms of Adaptation to Deformylase Inhibitors

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Anna Zorzet; Uppsala universitet.; [2010]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; antibiotic resistance; compensatory evolution; compensatory mutations; protein synthesis; initiation factor 2; initiator tRNA; formylation; peptide deformylase inhibitors; MEDICINE Microbiology; immunology; infectious diseases Microbiology Bacteriology; MEDICIN Mikrobiologi; immunologi; infektionssjukdomar Mikrobiologi Bakteriologi; Mikrobiologi; Microbiology; Biokemi; Biochemistry; Geography; Geografi;

    Sammanfattning : Antibiotic resistance is a growing problem on a global scale. Increasing numbers of bacteria resistant toward one or multiple antibiotics could return us to the high mortality rates for infectious diseases of the pre-antibiotic era. LÄS MER