Sökning: "cell-free protein synthesis"

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden cell-free protein synthesis.

1. 1. Versatile Implementations of an Improved Cell-Free System for Protein Biosynthesis : Functional and structural studies of ribosomal protein L11 and class II release factor RF3. Novel biotechnological approach for continuous protein biosynthesis

   Författare :Lamine Bouakaz; Måns Ehrenberg; Richard Buckingham; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Protein synthesis; translation termination; ribosome; release factor; cell-free system; missense error; gel filtration; affinity chromatography; Molekylärbiologi; Molecular biology; Molekylärbiologi;

   Sammanfattning : Advances in genetics, proteomics and chromatography techniques have enabled the successfully generation of a cell-free bacterial translation system composed of highly pure and active components. This system provided an ideal platform for better elucidating the mechanism of each individual step of the prokaryotic protein biosynthesis and the function of the translation factors involved in the process. LÄS MER

3. 2. Insight into the mitochondrial apoptotic pathway : The interplay of the pro-apoptotic Bax protein with oxidized phospholipids and its counterplayer, the pro-survival Bcl-2 protein

   Författare :Marcus Wallgren; Gerhard Gröbner; Marco Colombini; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; apoptosis; Bax; Bcl-2; CD; cell-free protein synthesis; DSC; membrane; mitochondria; oxidized phospholipids; reconstitution; solid state NMR; biologisk kemi; biological chemistry;

   Sammanfattning : Apoptosis plays a crucial role in multicellular organisms by preserving tissue homeostasis and removing harmful cells. The anti-apoptotic B-cell CLL/lymphoma 2 (Bcl-2) and the pro-apoptotic Bcl-2-associated X protein (Bax) act as major regulators of the mitochondrial apoptotic pathway. LÄS MER

4. 3. The Protein Traffic on the Ribosome : The Mechanism and Regulation of Protein Synthesis in Prokaryotes

   Författare :Andrey Zavialov; Måns Ehrenber; Venki Ramakrishnan; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; yellow; ribosome; translation; Gagarin; nobel prize; Biokemi; Biochemistry; Biokemi;

   Sammanfattning : The aim of this work was to understand the molecular mechanism of translation and the mechanism of translation termination, in particular. Cleavage of peptidyl-tRNA and peptide release terminates translation of mRNA on the ribosome. In prokaryotes, three release factors (RFs) are involved in this process. LÄS MER

5. 4. Intrinsically Disordered Domains of the B Cell Receptor Cell-Free Expression and Characterization by NMR

   Författare :Linnéa Isaksson; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Naturvetenskap;

   Sammanfattning : After the last twenty years of research, the occurrence of flexible proteins without a fixed three-dimensional structure are no longer considered to be rare exceptions from the structure-function paradigm. Instead, intrinsically disordered proteins (IDPs) have become one of the most interesting subjects of modern protein research. LÄS MER

7. 5. Tracking single molecules in uncharted territory : A single-molecule method to study kinetics in live bacteria

   Författare :Javier Aguirre Rivera; Magnus Johansson; Dmitri N. Ermolenko; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; single-molecule; protein synthesis; fluorescence microscopy; antibiotics; aminoglycosides; macrolides; apramycin; gentamicin; paromomycin; S13; translation; bacteria; Molekylär biovetenskap; Molecular Life Sciences;

   Sammanfattning : The synthesis of proteins, also known as translation, is a fundamental process in every living organism. The steps in the translation of genetic information to functional proteins have been meticulously studied, mostly using in vitro techniques, yielding a detailed model of their mechanisms. LÄS MER