Sökning: "cell immobilization"

Visar resultat 1 - 5 av 49 avhandlingar innehållade orden cell immobilization.

  1. 1. Microfluidic biosensing systems based on enzymes, antibodies and cells

    Författare :Richard Davidsson; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Analytisk kemi; chemiluminescence; immobilization; cell; antibody; enzyme; miniaturization; Microfluidic; microchip;

    Sammanfattning : The rapid developments within the life science and biotechnology areas put up ever-new demands on more sophisticated techniques and methods for chemical and biological analysis to reach deeper insight into the events at molecular level. Miniaturisation of assays and systems on microchips is one way to increase throughput and advance through parallel analysis lines, multiplexing and integration. LÄS MER

  2. 2. Affinity-, Partition- and Permeability Properties of the Human Red Blood Cell Membrane and Biomembrane Models, with Emphasis on the GLUT1 Glucose Transporter

    Författare :Christine Lagerquist Hägglund; Per Lundahl; Jørgen Vinten; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Affinity; Aromatic amino acids; Binding; Biomembrane; Biotin; Chromatography; Cytochalasin B; Dihydrocytochalasin B; Dissociation constant; Drug absorption; Ethanol; Equilibrium; Glucose; GLUT1; Immobilization; Immobilized biomembrane affinity chromatography; Immobilized liposome chromatography; Interaction; Liposome; Membrane protein; Membrane vesicle; Partitioning; Phospholipid bilayer; Proteoliposome; Quantitative; Red blood cell; Specific; Streptavidin; Tyrosine; Tryptophan; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods. LÄS MER

  3. 3. Quantitation, Purification and Reconstitution of the Red Blood Cell Glucose Transporter GLUT1

    Författare :Shusheng Zuo; Bengt Mannervik; Bengt Persson; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Cholesterol; Cytochalasin B; Glucose; GLUT1; Hummel and Dreyer analysis; Immobilization; PEG 5000 -DSPE; Biomembrane; Proteoliposome; Quantitative frontal affinity chromatography; Red blood cell streptavidin-biotin immobilization; Sulfhydryl affinity chromatography; The modified micro-Bradford CaPE assay; MALDI-ToF-MS; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : The human glucose transporter GLUT1 facilitates glucose to be accumulated on the other side of the cell membrane. The functional state of GLUT1 is uncertain due to diversity of the reports. In this thesis, the activity of red blood cell GLUT1 was extensively studied to further characterize this protein. LÄS MER

  4. 4. Design and characterization of direct electron transfer based biofuel cells including tests in cell cultures

    Författare :Peter Lamberg; Malmö högskola; []
    Nyckelord :biofuel cell; enzymatic fuel cell; direct electron transfer; cellobiose dehydrogenase; biocompatibility; enzyme kinetics; cell culture;

    Sammanfattning : Enzymatic fuel cells (EFCs) are bioelectronic devices based on redox enzymes, which convert chemical energy into electrical energy via biochemical reactions. A major difficulty to overcome is to successfully connect (using e.g., immobilization) the enzymes to the electrode surface. LÄS MER

  5. 5. Methods for cell line and protein engineering

    Författare :Magnus Lundqvist; Johan Rockberg; Mathias Uhlén; Diethard Mattanovich; KTH; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell line development; therapeutic proteins; protein engineering; molecular cloning; mutagenesis; split-GFP; Biotechnology; Bioteknologi;

    Sammanfattning : Therapeutic proteins are becoming increasingly important. They are desirable, as they typically possess low adverse effects and higher specificity compared to the traditional, small molecule drugs. But they are also more complex and involve different intricate and expensive development and production processes. LÄS MER