Sökning: "cell immobilization"
Visar resultat 1 - 5 av 50 avhandlingar innehållade orden cell immobilization.
1. Microfluidic biosensing systems based on enzymes, antibodies and cells
Sammanfattning : The rapid developments within the life science and biotechnology areas put up ever-new demands on more sophisticated techniques and methods for chemical and biological analysis to reach deeper insight into the events at molecular level. Miniaturisation of assays and systems on microchips is one way to increase throughput and advance through parallel analysis lines, multiplexing and integration. LÄS MER
2. Quantitation, Purification and Reconstitution of the Red Blood Cell Glucose Transporter GLUT1
Sammanfattning : The human glucose transporter GLUT1 facilitates glucose to be accumulated on the other side of the cell membrane. The functional state of GLUT1 is uncertain due to diversity of the reports. In this thesis, the activity of red blood cell GLUT1 was extensively studied to further characterize this protein. LÄS MER
3. Design and characterization of direct electron transfer based biofuel cells including tests in cell cultures
Sammanfattning : Enzymatic fuel cells (EFCs) are bioelectronic devices based on redox enzymes, which convert chemical energy into electrical energy via biochemical reactions. A major difficulty to overcome is to successfully connect (using e.g., immobilization) the enzymes to the electrode surface. LÄS MER
4. Affinity-, Partition- and Permeability Properties of the Human Red Blood Cell Membrane and Biomembrane Models, with Emphasis on the GLUT1 Glucose Transporter
Sammanfattning : The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods. LÄS MER
5. Chromatographic Studies of Solute Interactions with Immobilized Red Blood Cells and Biomembranes
Sammanfattning : Specific and non-specific interactions of solutes with immobilized biomembranes were studied using chromatographic methods. Liposomes, proteoliposomes and red blood cell (RBC) membrane vesicles were immobilized by a freeze-thawing procedure, whereas whole RBCs were adsorbed in the gel beds using electrostatic interaction, binding to wheat germ agglutinin (WGA) or the streptavidin-biotin interaction. LÄS MER