Sökning: "cell factories"

Visar resultat 1 - 5 av 32 avhandlingar innehållade orden cell factories.

  1. 1. Droplet microfluidics for screening and sorting of microbial cell factories

    Författare :Sara Björk; Helene Svahn Andersson; Håkan Jönsson; Christoph Merten; KTH; []
    Nyckelord :Droplet microfluidics; Cell factories; High-throughput screening; Cell culture; Biotechnology; Bioteknologi;

    Sammanfattning : Cell factories are cells that have been engineered to produce a compound of interest, ranging from biopharmaceuticals to biofuels. With advances in metabolic engineering, the number of cell factory variants to evaluate has increased dramatically, necessitating screening methods with increased throughput. LÄS MER

  2. 2. Droplet microfluidics for single cell and nucleic acid analysis

    Författare :Prem Kumar Periyannan Rajeswari; Helene Andersson Svahn; Håkan Jönsson; Martin Dufva; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Biomarker detection; Cell behavior analysis; Cell factories; Droplet microfluidics; Droplet PCR; High throughput biology; Label-free enrichment; Single cell analysis; Biotechnology; Bioteknologi;

    Sammanfattning : Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at high throughput. The controlled encapsulation of particles along with the surrounding microenvironment in discrete droplets, which acts as miniaturized reaction vessels, allows millions of particles to be screened in parallel. LÄS MER

  3. 3. Engineering microbial cell factories for protein production

    Författare :Kiavash Mirzadeh; Daniel Daley; Grzegorz Kudla; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Protein production; expression vector; recombinant DNA; Translation initiation region; Escherichia coli; mRNA secondary structure; Synthetic evolution; biokemi; Biochemistry;

    Sammanfattning : Proteins are often produced using microbial cell factories for academic or industrial purposes. Protein production is however not an open-and-shut procedure. Production yields often vary in an unpredictable and context dependent manner, limiting the rational design of a straightforward production experiment. LÄS MER

  4. 4. Systems Biology of the Secondary Metabolism in Filamentous Fungi

    Författare :Jens Christian Froslev Nielsen; Chalmers University of Technology; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; genomics; systems biology; secondary metabolites; antibiotics; Next-generation sequencing; secondary metabolism; filamentous fungi; cell factories; Penicillium;

    Sammanfattning : Filamentous fungi constitute a rich reservoir of pharmaceutically relevant bioactive small molecules. These compounds, commonly referred to as secondary metabolites, are widely used as antibiotics for the treatment of microbial infections, but also as other pharmaceuticals such as immunosuppressors, cholesterol lowering agents and anticancer drugs. LÄS MER

  5. 5. Understanding and improving microbial cell factories through Large Scale Data-approaches

    Författare :Daniel Brink; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microbiology; lignocellulose; lignin; D-xylose; Saccharomyces cerevisiae; Pseudomonas putida; bioinformatics; genome assembly; flow cytometry; metabolic engineering; sugar sensing signalling;

    Sammanfattning : Since the advent of high-throughput genome sequencing methods in the mid-2000s, molecular biology has rapidly transitioned towards data-intensive science. Recent technological developments have increased the accessibility of omics experiments by decreasing the cost, while the concurrent design of new algorithms have improved the computational work-flow needed to analyse the large datasets generated. LÄS MER