Sökning: "carbohydrate-active enzyme"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden carbohydrate-active enzyme.

1. 1. Structural and functional investigation of underexplored carbohydrate-active enzyme families

   Författare :Andrea Seveso; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; carbohydrate-active enzymes; lytic polysaccharide monooxygenase; plant biomass degradation; copper radical oxidases; carbohydrate esterase; protein structure determination; protein structure prediction;

   Sammanfattning : The known consequences of the current fossil-based economy require a transition towards a bio-based economy. Development of biorefineries in which plant biomass can be utilized as a renewable source of energy and building blocks to produce both commodities and high-value products, is a key step in this transition. LÄS MER

3. 2. Production and investigation of highly thermophilic multi-domain carbohydrate-active enzymes

   Författare :Daniel Krska; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; protein structure; xylanase; carbohydrate esterase; multidomain enzymes; carbohydrate-active enzymes; thermostable enzymes; plant biomass degradation; Caldicellulosiruptor;

   Sammanfattning : With the looming threat of climate change caused largely by an excess of carbon dioxide in the atmosphere, recent scientific efforts have focused on the substitution of fossil fuels and other polluting compounds with more environmentally conscious choices. To this end, the investigation of biomass as both a renewable source of energy and as a chemical basis to produce high-value products is being extensively investigated. LÄS MER

4. 3. Feruloyl esterases - Evaluation of their potential for biotechnological applications

   Författare :Cyrielle Bonzom; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; multi-domain enzyme; acetyl esterase; enzyme immobilization; N-glycosylation; carbohydrate active enzyme family 1; acetyl xylan esterase; heterologous production; polysaccharide utilization loci; enzyme stability; feruloyl esterase;

   Sammanfattning : Owing to the current efforts to find sustainable alternatives to petrochemical based industries and technologies, enzymatic degradation and valorization of plant biomass has been attracting interest. Due to the complexity of plant biomass, an array of enzymes is required to hydrolyze it, including esterases. LÄS MER

5. 4. Discovery and applications of family AA9 lytic polysaccharide monooxygenases

   Författare :Monika Tõlgo; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lytic polysaccharide monooxygenases; cellulose; xylan; spruce; carbohydrate active enzymes; LPMO; lignocellulolytic enzyme cocktails; lignocellulose; polysaccharides; AA9; cellulose nanocrystals; thermophilic fungi;

   Sammanfattning : Auxililary activity family 9 lytic polysaccharide monooxygenases (abbreviated as AA9s or LPMO9s) are fungal mono-copper enzymes capable of oxidatively cleaving various plant cell wall oligo- and/or polysaccharides. LPMO9s are key components of lignocellulolytic enzyme cocktails used in today’s biorefineries to break down biomass into fermentable sugars. LÄS MER

7. 5. On the engineering of proteins: methods and applications for carbohydrate-active enzymes

   Författare :Fredrika Gullfot; Harry Brumer; Antoni Planas; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; enzyme engineering; rational design; directed evolution; DNA shuffling; glycosynthase; xyloglucan; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; xyloglucanase; carbohydrate binding module; polysaccharide synthesis; Bioengineering; Bioteknik;

   Sammanfattning : This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. LÄS MER