Sökning: "cDNA selection"

Visar resultat 6 - 10 av 23 avhandlingar innehållade orden cDNA selection.

  1. 6. Functional aspects on the Epstein-Barr virus nuclear antigen 5

    Författare :Martin Dufva; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Epstein-Barr Virus; EBV; B-cell; transformation; cDNA array; polyadenylation; transcription; cell cycle; apoptosis; two-hybrid system.;

    Sammanfattning : The Epstein-Barr virus (EBV) infects resting B-cells. This leads to an EBV driven transformation of the resting B-cell into a cell with an activated B-cell phenotype that proliferates indefinitely in vitro. Epstein-Barr Nuclear antigen 5 (EBNA5) is one out of six EBV encoded protein involved in transformation of resting B-cell. LÄS MER

  3. 7. Redesign of substrate specificity of glutathione transferase and glutathione reductase : Enzyme engineering by directed mutagenesis, phage-display selection and DNA shuffling

    Författare :Lars O. Hansson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Human glutathione transferase (GST) A1-1 was expressed in fusion with the phage gene IIIproduct and a library of phage-displayed GST mutants was constructed by randomization of fouractive-site residues. Variant GSTs were isolated by mechanism-based phage display selection, using an immobilized transition-state analog of a nucleophilic aromatic substitution (SNAr) reaction. LÄS MER

  4. 8. Molecular studies of multiple endocrine neoplasia type 1 (MEN1)

    Författare :Shideh Khodaei-O'Brien; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :genetics; MEN1; cancer; tumor suppressor gene; cDNA selection; mutation; menin; ortholog; evolution; conservation; expression; RNA; protein; RT-PCR;

    Sammanfattning : Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant familial cancer syndrome characterized by tumors of the parathyroids, the endocrine pancreas and anterior pituitary. The MEN1 locus has been previously localized to chromosome 11q13 and subsequently a MEN1 minimum region was defined by a combination of linkage and tumor deletion studies. LÄS MER

  5. 9. Expression of recombinant neutralizing anti-HIV-1 antibodies in bacteria and eukaryotic cells

    Författare :Fayezeh Yari; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Phage display; vector; monoclonal antibody; antigen; eukaryotic; prokaryotic; transfection; selection; transient expression; stable cell line; neutralizing antibody; expression; HIVA-1; gp41; gp120;

    Sammanfattning : The main purpose of this study was to investigate and optimize techniques for eukaryotic expression of antibody molecules (IgG, Fab and scFV-ê formats), derived from an immune phage displayed Fab library and reactive against HIV-1 glycoproteins gp120 and gp41. Light and heavy-chain (Fd) DNA from a number of Fab clones were transferred from a prokaryotic phagemid vectors to mammalian expression vectors. LÄS MER

  7. 10. Exploring the Realm of Gene Expression Differences Between White Leghorn and Red Junglefowl Chickens

    Författare :Carolyn Fitzsimmons; Leif Andersson; Finn Hallböök; Dave Burt; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular genetics; chicken; gene expression; microarray; polymorphism; Genetik; Genetics; Genetik;

    Sammanfattning : In this thesis we attempted to elicit patterns of gene expression that influence phenotype, and that may also have been altered by thousands of years of domestication and selection, between red junglefowl and White Leghorn chickens. Red junglefowl are the wild ancestor to all domesticated chickens, and poultry in general are highly valued as a research animal and food resource. LÄS MER