Sökning: "cDNA differential display"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden cDNA differential display.

  1. 1. Identification of abnormally expressed genes in skeletal muscle contributing to insulin resistance and type 2 diabetes

    Författare :Xudong Huang; Malmö Institutionen för kliniska vetenskaper; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Endocrinology; secreting systems; diabetology; Endokrinologi; gene expression; high-fat intake; RT-PCR; mitochondrial genes; cathepsin L; Shc; IRS-2; IRS-1; GS; cDNA differential display; Insulin resistance; type 2 diabetes; sekretion; diabetologi;

    Sammanfattning : The metabolic defects of insulin resistance and type 2 diabetes can result from changes in gene expression and protein functions due to genetic and environmental influences. The aim of this study was to identify abnormally expressed genes associated with insulin resistance or type 2 diabetes, and further to test whether possible defects are inherited or acquired. LÄS MER

  3. 2. Molecular mechanisms involved in glioma cell interactions in vitro and studies of PDGF B transcript variants

    Författare :Susanne Heller; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Tumor heterogeneity; glioma; differential display; PDGF B; Genetik; Clinical genetics; Klinisk genetik; Pathology; patologi;

    Sammanfattning : Glioblastoma multiforme is a malignant brain tumor characterized by heterogeneity.Interactions between heterogeneous tumor cells are supposed to affect the behavior of awhole tumor cell population. LÄS MER

  4. 3. Redesign of substrate specificity of glutathione transferase and glutathione reductase : Enzyme engineering by directed mutagenesis, phage-display selection and DNA shuffling

    Författare :Lars O. Hansson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Human glutathione transferase (GST) A1-1 was expressed in fusion with the phage gene IIIproduct and a library of phage-displayed GST mutants was constructed by randomization of fouractive-site residues. Variant GSTs were isolated by mechanism-based phage display selection, using an immobilized transition-state analog of a nucleophilic aromatic substitution (SNAr) reaction. LÄS MER

  5. 4. Innate immune gene expression and function studied in insects and mammals

    Författare :Gang Liu; Ulrich Theopold; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mikrobiologi; Microbiology;

    Sammanfattning : Insects and mammals possess innate immune responses to effectively recognize and eliminate invading microorganisms. Insects, who share with mammals conserved molecules for immune defense, are good model systems for innate immunity studies. LÄS MER

  7. 5. Biotic and abiotic stress in microorganisms and plants: Some Mechanisms of Stress and Programmed Cell Death. Genetic Engineering Approaches Towards Improved Stress Tolerance

    Författare :Per-Johan Meijer; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; biotic stress; plants; microorganisms; stress tolerance; antifreeze protein; proline; programmed cell death; systemic aquired resistance; Biochemistry; metabolism; Biokemi; Bioteknik; Biotechnology; abiotic stress;

    Sammanfattning : Various forms of stress, such as biotic and abiotic stress, were studied in microorganisms and plants to learn more about the underlying mechanisms of such stress and potential genetic engineering strategies towards stress tolerance. An antifreeze protein from winter flounder, expressed in E. LÄS MER