Sökning: "biotinylation"

Visar resultat 1 - 5 av 12 avhandlingar innehållade ordet biotinylation.

1. 1. RADIOIMMUNOTHERAPY & EXTRACORPOREAL ADSORPTION Preclinical Studies of the Pharmacokinetics & Biotinylation of Radioimmunoconjugates in Immunocompetent Rats

   Författare :Zhongmin Wang; Bröstcancer-genetik; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; onkologi; cancer; oncology; cancerology; Cytologi; Cytology; extracorporeal adsorption; tri-functional chelator; maximal tolerable dose; biotin; intraperitoneal administration; biodistribution; pharmacokinetics; biotinylation; radiolabeling; rat; monoclonal antibody; radionuclide;

   Sammanfattning : Extracorporeal adsorption (ECAT) is a novel strategy to reduce activity in whole body (WB), blood and radiosensitive organs by removing the excess of radiolabeled and biotinylated monoclonal antibody (MAb) from blood so that tumor/normal tissue ratio (T/N) could be improved. By increasing T/N, higher amounts of radiolabeled MAb might be administered, making it possible to treat disseminated carcinomas. LÄS MER

3. 2. Radiolabeling and Biotinylation of Internalizing Monoclonal Antibody Chimeric BR96: Potential Use for Extracorporeal Immunoadsorption

   Författare :Jianqing Chen; Lund Medicinsk strålningsfysik; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; serologi; Immunologi; transplantation; Immunology; serology;

   Sammanfattning : In this thesis, methodology of radiolabeling and simultaneous biotinylation for internalizing monoclonal antibody (MAb) chimeric BR96 (chiBR96) have been investigated by using three element groups of potential therapeutic radionuclides iodine, indium and rhenium, and their different labeling methods. The biodistribution and kinetics of biotinylated and radiolabeled chiBR96 have been studied in colon carcinoma isografted rats. LÄS MER

4. 3. Validation of antibodies for tissue based immunoassays

   Författare :Sandra Andersson; Anna Asplund; Fredrik Pontén; Kenneth Wester; Mike Taussig; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody validation; Conjugation; Estrogen receptor-beta; IHC; ISH; Lymphohematopoietic tissues; Proteomic; RNAseq; TMA; Transcriptomic; Patologi; Pathology;

   Sammanfattning : In situ protein detection in human tissues using antibodies reveals the cellular protein localization, and affinity-based proteomic studies can help to discover proteins involved in the development of diseases. However, antibodies often suffer from cross-reactivity, and the lack of positive and negative tissue controls for uncharacterized proteins complicates the mapping of the proteome. LÄS MER

5. 4. Proteomics as a multifaceted tool in medicine and environmental assessment

   Författare :Jacob Kuruvilla; Susana Cristobal; Mats Lindahl; Mikael Sigvardsson; Jean Armengaud; Linköpings universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

   Sammanfattning : Proteomics is evolving as a multi-faceted tool for addressing various biochemical and biomedical queries in the field of scientific research. This involves various stages, ranging from sample preparation to data analysis and biological interpretation. LÄS MER

7. 5. Generating Affinity Proteins for Biotechnological, Diagnostic and Therapeutic Applications

   Författare :Feifan Yu; Per-Åke Nygren; Lynne Regan; KTH; []
   Nyckelord :Protein engineering; Bioteknologi; Biotechnology;

   Sammanfattning : Protein engineering is a powerful tool to modify proteins to generate novel and desired properties that could be applied in biotechnological, diagnostics and therapeutic areas. In this thesis, both rational design and library based engineering principles have been exploited to develop affinity proteins with desired traits. LÄS MER