Sökning: "biological cloning"

Visar resultat 1 - 5 av 58 avhandlingar innehållade orden biological cloning.

  1. 1. Functional studies of plant hexokinases and development of genetic methods in the moss Physcomitrella patens

    Författare :Mikael Ulfstedt; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES;

    Sammanfattning : In the field of biology, model systems are frequently used for practical reasons. Model organisms possess several features that make them easy to work with in a laboratory setting. In addition they usually have a host of established genetic tools that have been developed by the research community. LÄS MER

  3. 2. CRISPR based technologies for high-throughput metabolic engineering of yeast

    Författare :Christos Skrekas; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cloning; Saccharomyces cerevisiae; enrichment; metabolic engineering; selection; library; screening; CRISPR; directed evolution;

    Sammanfattning : The yeast Saccharomyces cerevisiae is a commonly used microorganism for metabolic engineering applications since it has a very well-studied metabolism and it can be easily genetically modified. This led to its use as a cell factory for the production of a wide variety of industrially relevant chemicals such as fuels, cosmetics and food additives. LÄS MER

  4. 3. Galactoglucomannan-degrading enzymes from Aspergillus niger

    Författare :Pia Ademark; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; beta-mannanase; Aspergillus niger; beta-mannosidase; alpha-galactosidase; purification; hydrolysis; cloning; galactoglucomannan; expression; Biochemistry; Metabolism; Biokemi;

    Sammanfattning : Galactoglucomannan and galactomannan are among the most abundant plant polysaccharides in nature. The central enzymes involved in their degradation, i.e., endo-1,4-beta-mannanase (beta-mannanase, EC 3. LÄS MER

  5. 4. Sesquiterpene synthases in Artemisia annua: Cloning, Expression, and Characterisation of the Recombinant Enzymes

    Författare :Per Mercke; Kemiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Artemisia annua; Sesquiterpene synthases; artemisinin; Plant biochemistry; Växtbiokemi;

    Sammanfattning : Plants produce a multitude of different compounds that may have biotic effects. Artemisia annua, produces a compound used as a drug against multi-drug-resistant strains of Plasmodium falciparum, the parasite causing malaria. LÄS MER

  7. 5. Regulation of Hsp70 function by nucleotide-exchange factors

    Författare :Naveen Kumar Chandappa Gowda; Claes Andréasson; Gary Jones; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Heat shock proteins Hsps ; Hsp70; Molecular chaperones; Nucleotide-exchange factors NEFs ; Protein quality control PQC ; Proteostasis; Ubiquitin-proteasome system; Cell Biology; cellbiologi;

    Sammanfattning : Protein folding is the process in which polypeptides in their non-native states attain the unique folds of their native states. Adverse environmental conditions and genetic predisposition challenge the folding process and accelerate the production of proteotoxic misfolded proteins. LÄS MER