Sökning: "batch effect"

Visar resultat 1 - 5 av 107 avhandlingar innehållade orden batch effect.

  1. 1. Physiology of Escherichia coli in batch and fed-batch cultures with special emphasis on amino acid and glucose metabolism

    Författare :Ling Han; KTH; []
    Nyckelord :escherichia coli; amino acids; glycine; quorum sensing; metabolic switch; metabolite po9ols; carbon balance; outer membrne; lipopolysaccharide; batch culture;

    Sammanfattning : The objective of this work is to better understand themetabolism and physiology ofEscherichiacoli(W3110) in defined medium cultures with thelong-term goal of improving cell yield and recombinant proteinproductivity.The order of amino acid utilization inE. colibatch cultures was investigated in a medium with16 amino acids and glucose. LÄS MER

  3. 2. Interpretation of variation in omics data : Applications in proteomics for sustainable agriculture

    Författare :Jakob Willforss; Institutionen för immunteknologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; agriculture; proteomics; omics; biomarker; normalization; batch effect; visualization; software;

    Sammanfattning : Biomarkers are used in molecular biology to predict characteristics of interest and are applied in agriculture to accelerate the breeding of target traits. Proteomics has emerged as a promising technology for improved markers by providing a closer view to the phenotype than conventional genome-based approaches. LÄS MER

  4. 3. Bioelectronic Nanosensor Devices for Environmental and Biomedical Analysis

    Författare :Klas Risveden; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Regional Ion Sensitive Field Effect Transistor; Region Ion Sensitive Field Effect Transistor; RISFET; Conducting channel; Bioelectronic Nanosensor; Bioelectronic; Biosensor; Nanosensor; Chemical Sensor; Sensor; Semiconductor sensor; Single molecule trapping; Scaling; Protein trapping; Nanoelectronics; Lab-on-a-chip; pico-ammeter; Nanobiosensor; Sample Applicator; Sequential Batch Analysis; Sequential Batch Analysis System; SBAS; Nanowire; Branched nanowire structure; Nanotree; Nanorod; Nano processing; Micro processing; Electron Beam Lithography; EBL; UV-lithography; Molecular Imprinting; DUV-lithography; Moleculary Imprinted Polymers; MIP; Biomimetics; QCM; Biomimetic sensor; Quartz Crystal Microbalance; QCM-D; Nanoparticles; Acetylcholine esterase; Glucose oxidase; Glucose; Gluconolactone; Propranolol; Gluconate; Carbofuran; Neurotoxic; Environmental analysis; Biomedical Analysis; Biotechnology; Medicine; Pesticide; Food Technology; Analyis; Flow Injection Analysis; FIA; Scanning Probe Microscopy; Scanning Kelvin Probe Microscopy; SPM; KPM; AFM; SKM; KPFM; SKPM; Nanostructures.; Atomic Force Microscopy; KFM;

    Sammanfattning : A new type of Bioelectronic Nanosensor Device with potential applications in medicine,biotechnology and environmental analysis was designed. The nanosensor is based on RISFET (Regional Ion Sensitive Field Effect Transistor) technology. LÄS MER

  5. 4. Modeling and Simulation of a Multi Phase Semi-batch Reactor

    Författare :Anna Nyström; Göteborgs universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; semi-batch reactor; modeling; simulation; optimization; simulation;

    Sammanfattning : The operation of an industrial semi-batch reactor is modeled and the flow of one reactant is investigated. In the reactor a strongly exothermic polymerization reaction takes place followed by a slightly exothermic reaction, and the objective is to minimize the duration of the operation of the process. LÄS MER

  7. 5. Development of a protein-free fed-batch process for NS0 cells: studies on regulation of proliferation

    Författare :Erika Spens; Lena Häggström; Jean-Louis Goergen; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NS0 myeloma cells; protein-free medium; fed-batch; conditioned medium; autocrine growth factors; abortive proliferation; protease activity; aprotinin; gp130; Bioengineering; Bioteknik;

    Sammanfattning : The overall objective of this study was to investigate how NS0 cell proliferation is regulated in protein-free media. The hypothesis was that during the adaptation to growth factor-free media, animal cell lines start to produce their own autocrine growth factors to support proliferation, and after some time in a culture the effects of these factors are lost which results in cessation of proliferation. LÄS MER