Sökning: "batch cultivations"

Visar resultat 1 - 5 av 31 avhandlingar innehållade orden batch cultivations.

  1. 1. Characterization of respiratory physiology in Lactococcus lactis for high-yield production of high-performance starter cultures

    Författare :Anna Johanson; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; batch cultivations; lag phase; haemin haem; respiration-permissive conditions; aerobic growth; Lactic acid bacteria; acidification activity; continuous cultivations; starter culture production;

    Sammanfattning : Fermented food products are consumed world-wide, every day, and the demand is growing as intake increases. Lactococcus lactis is a lactic acid bacterium commonly used as a starter culture to produce fermented dairy products. LÄS MER

  3. 2. Bioprocess Monitoring and Control in Pseudomonas cepacia and recombinant Escherichia coli cultivations

    Författare :Anita Tocaj; LTH Ingenjörshögskolan vid Campus Helsingborg; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein L; expanded bed; flow-injection analysis; recombinant protein production; acetic acid; on-line measurements; Pseudomonas cepacia; recombinant Escherichia coli; Fed-batch cultivations; control strategies; xylanase deletion derivatives from Rhodothermus marinus; Biotechnology; Bioteknik;

    Sammanfattning : The control of cultivation conditions and its influence on growth and production have been investigated. The use of fed-batch processes have been demonstrated for several cultivation systems and so has the use of different control signals in these systems. LÄS MER

  4. 3. Probing Control of Glucose Feeding in Escherichia coli Cultivations

    Författare :Mats Åkesson; Institutionen för reglerteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Automatiska system; control engineering; robotics; Automation; dissolved oxygen; acetate; glucose feeding; robotteknik; Escherichia coli; Process control; fed-batch cultivation; reglerteknik; Biotechnology; Bioteknik;

    Sammanfattning : Production of many proteins can today be made using genetically modified organisms. One of the most frequently used host organisms is the bacterium Escherichia coli. A difficulty encountered in cultivations of E. coli is the accumulation of the metabolic by-product acetate which inhibits cell growth and production of a desired protein. LÄS MER

  5. 4. Feeding Strategies Based on Probing Control for E. coli and V. cholerae Cultivations

    Författare :Lena de Maré; Institutionen för reglerteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; robotics; control engineering; Automatiska system; robotteknik; reglerteknik; Automation; Temperature; Mid-ranging control; Amino acid; Complex media; Fed-batch bioreactors; Probing control; Biotechnology; Bioteknik; Parameter estimation; Feeding strategies;

    Sammanfattning : The recombinant DNA techniques have made it possible to produce many different proteins for a wide range of applications. The vector encoding for the recombinant protein is often inserted in the bacterium E. coli as it is a well studied and a well-known organism. To achieve a high productivity, it is important to reach a high cell density. LÄS MER

  7. 5. Growth rate control of periplasmic product retention in Escherichia coli

    Författare :Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

    Sammanfattning : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. LÄS MER