Sökning: "automated microscopy"

Visar resultat 1 - 5 av 58 avhandlingar innehållade orden automated microscopy.

  1. 1. Towards subcellular localization of the human proteome using bioimaging

    Författare :Charlotte Stadler; Emma Lundberg; Carolina Wählby; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; antibody validation; automated image analysis; automated microscopy; cell line; confocal microscopy; fixation; green fluorescent protein GFP ; immunofluorescence IF ; organelle; protein expression; siRNA; subcellular localization;

    Sammanfattning : Since the publication of the complete sequence of the human genome in 2003 there has been great interest in exploring the functions of the proteins encoded by the genes. To reveal the function of each and every protein, investigation of protein localization at the subcellular level has become a central focus in this research area, since the localization and function of a protein is closely related. LÄS MER

  3. 2. Automated super-resolution microscopy for high-throughput imaging

    Författare :Xavier Casas Moreno; Ilaria Testa; Florian Jug; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RESOLFT; throughput; microscopy automation; nanoscopy; Biologisk fysik; Biological Physics;

    Sammanfattning : Fluorescence microscopes enable the visualization of biological samples with high contrast by labeling specific structures with fluorescent molecules. However, the spatial resolution of widely used microscopy techniques, such as widefield and confocal microscopy, is limited by the size of a focused spot of light, which roughly corresponds to half the wavelength of the illumination. LÄS MER

  4. 3. Automated Photoluminescence Experimentation for Understanding Dynamic Metal-Halide Perovskite Semiconductors

    Författare :Alexander Kiligaridis; Kemisk fysik; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Photoluminescence; Perovskites; Semiconductor; Spectroscopy; Microscopy;

    Sammanfattning : Metal halide perovskites have garnered significant attention for their remarkable optoelectronic properties and unique photophysical characteristics. Combined with low cost for fabrication, this new class of materials is among the most promising candidates within next generation photovoltaic technologies. LÄS MER

  5. 4. Deep Neural Networks and Image Analysis for Quantitative Microscopy

    Författare :Sajith Kecheril Sadanandan; Carolina Wählby; Petter Ranefall; Ewert Bengtsson; Jeroen van der Laak; Uppsala universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Deep neural networks; convolutional neural networks; image analysis; quantitative microscopy; bright-field microscopy; Datoriserad bildbehandling; Computerized Image Processing;

    Sammanfattning : Understanding biology paves the way for discovering drugs targeting deadly diseases like cancer, and microscopy imaging is one of the most informative ways to study biology. However, analysis of large numbers of samples is often required to draw statistically verifiable conclusions. LÄS MER

  7. 5. Statistical methods in single particle fluorescence microscopy

    Författare :Magnus Röding; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; absolute number concentration; Brownian motion; diffusion; fluorescence microscopy; image analysis; image processing; nanoparticle characterization; particle tracking; Smoluchowski process; nanoparticle characterization;

    Sammanfattning : The aim of this thesis is stochastic modeling and statistical inference in single particle fluorescence microscopy related to the experimental observation of randomly moving particles. A central theme is the use of single particle microscopy for estimation of absolute concentration of nanoparticles performing Brownian motion. LÄS MER