Sökning: "antisense RNA"

Visar resultat 16 - 20 av 69 avhandlingar innehållade orden antisense RNA.

  1. 16. Chemically Modified Oligonucleotides: Synthesis, Physicochemical and Biochemical Properties of their Duplexes with DNA and RNA

    Författare :Pushpangadan Indira Pradeepkumar; Jyoti Chattopadhyaya; Jesper Wengel; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; Antisense oligonucleotides; Dipyridophenazine; Oxetane; RNase H; Gene down-regulation; DNase 1; Charge transport; Bioorganisk kemi; Bioorganic chemistry; Bioorganisk kemi;

    Sammanfattning : This thesis is based on 9 papers dealing with the synthesis, physicochemical and biochemical properties of two types of chemically modified oligonucleotides which have the potential to down-regulate gene expression: (i) The first set is comprised of antisense oligonucleotides (AONs) conjugated with different chromophores of varying size, charge and π-electron density. Conjugation of the chromophores at the 3'- or 5'-end enhanced the target RNA binding affinity and RNase H recruitment capabilities compared to the native counterpart without changing the global helical conformation of their AON/RNA hybrid duplexes. LÄS MER

  3. 17. The Involvement of the structures of antisense RNA, target RNA, and their complexes in plasmid R1 copy number regulation

    Författare :Charlotta Ingvoldstad; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; mikrobiologi; Microbiology;

    Sammanfattning : Plasmid R1 is a low-copy-number plasmid which belongs to the incompatibility group FII. R1 regulates its own copy number. Initiation of replication of plasmid R1 requires the binding of the RepA protein to oriR1. RepA acts in cis and has to be synthesized de novo for each round of replication. LÄS MER

  4. 18. An investigation of structural determinants that affect the efficiency of antisense RNA control

    Författare :Tord Å. H. Hjalt; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : .... LÄS MER

  5. 19. Studies on natural antisense RNAs and microRNAs

    Författare :Omid Reza Faridani; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Regulatory RNAs are found in all kingdoms of life and involved in regulation of gene expression at various steps including RNA splicing, editing, stability, modification, export, translation and chromatin remodelling. A large number of regulatory RNAs have been described recently. LÄS MER

  7. 20. Novel Pentofuranose Chemistry to Modulate RNA Function

    Författare :Mansoureh Karimiahmadabadi; Jyoti Chattapadhyaya; Jesper Wengel; Uppsala universitet; []
    Nyckelord :pentofuranose chemistry; conformationally locked nucleoside; carbocyclic-LNA; bicyclo[2.2.1]heptane;

    Sammanfattning : Chemical modifications of oligonucleotides provide an important tool to understand how the natural substrate works as well as how to improve their biochemical and biological properties as potential therapeutics and diagnostics. Our carba-LNA (2',4'-carba-bridged Locked Nucleic Acid) modified oligo-DNA or -RNA have been found to be useful to modulate oligo-RNA and -DNA activity. LÄS MER