Sökning: "antibody-based proteomics"

Visar resultat 16 - 20 av 25 avhandlingar innehållade orden antibody-based proteomics.

1. 16. Multiplexed absolute quantification of blood plasma proteins using targeted proteomics

   Författare :David Kotol; Mathias Uhlén; Edfors Fredrik; Roman Zubarev; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mass spectrometry; targeted proteomics; stable isotope standards; recombinant protein standards; absolute quantification; blood plasma; multiplex analysis; plasma profiling; Biotechnology; Bioteknologi;

   Sammanfattning : Proteins are the molecular building blocks of all living organisms. They are the functioning actors in metabolism and communication, and they give specific architecture and purpose to all tissues. Their abundance is dynamic and can be interpreted and associated to different physiological states or diseases. LÄS MER

3. 17. Discovering circulating protein biomarkers through in-depth plasma proteomics

   Författare :Haris Babačić; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Plasma, i.e., the liquid component of blood, is one of the most clinically used samples for biomarker measurement. LÄS MER

4. 18. The spatiotemporal protein landscape of human cells

   Författare :Lovisa Stenström; Emma Lundberg; Björn Högberg; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Spatial proteomics; Immunofluorescence; Human Protein Atlas; Nucleolus; Cell cycle; Citizen science; Bioteknologi; Biotechnology;

   Sammanfattning : This thesis focuses on the spatiotemporal mapping of proteins at a subcellular level. In other words, determining the cellular location of proteins over time. From a biological point of view, knowledge about protein location is fundamental to understand protein function. LÄS MER

5. 19. Bioimaging for analysis of protein expression in cells and tissues using affinity reagents

   Författare :Emma Lundberg; Torbjörn Gräslund; Mathias Uhlén; Rainer Pepperkok; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Affibody; antibody; biomarker; cell line; cell microarray; colorectal cancer; confocal microscopy; HER2; immunohistochemistry; immunofluorescence; light microscopy; protein expression; protein localization; SATB2; tissue microarray; Bioengineering; Bioteknik; Cell and molecular biology; Cell- och molekylärbiologi;

   Sammanfattning : The detection and analysis of biomolecules, such as proteins, are of great interest since these molecules are fundamental for life and our health. Due to the complexity of biological processes, there is a great advantage of studying proteins in their natural context, for example by using bioimaging. LÄS MER

7. 20. Design of recombinant antibody microarrays for global proteome analysis

   Författare :Johan Ingvarsson; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bioteknik; oncoproteomics; Recombinant antibody microarray; scFv; Biotechnology; plasma proteome analysis;

   Sammanfattning : Antibody-based microarrays are among the new, rapidly emerging technologies within the field of proteomics that has the potential to evolve into a key proteomic research tool, providing novel means to perform early diagnostics, identify biomarkers and gain insights into disease biology. The aim of this thesis, based on five original papers, was to develop antibody microarrays based on human recombinant scFv antibody fragments for large-scale comprehensive proteome analysis. LÄS MER