Sökning: "antibody-based measurements"

Hittade 4 avhandlingar innehållade orden antibody-based measurements.

  1. 1. Antibody-based Profiling of Expression Patterns using Cell and Tissue Microarrays

    Författare :Sara Strömberg; Fredrik Ponten; Joakim Lundeberg; Anna Asplund; Stephen Hewitt; Uppsala universitet; []
    Nyckelord :Pathology; antibody-based proteomics; tissue microarray; cell line; immunohistochemistry; melanocytes; image analysis; biomarker; Patologi;

    Sammanfattning : In this thesis, methods to study gene and protein expression in cells and tissues were developed and utilized in combination with protein-specific antibodies, with the overall objective to attain greater understanding of protein function.To analyze protein expression in in vitro cultured cell lines, a cell microarray (CMA) was developed, facilitating antibody-based protein profiling of cell lines using immunohistochemistry (IHC). LÄS MER

  3. 2. Towards spatial host-microbiome profiling

    Författare :Britta Lötstedt; Joakim Lundeberg; Petri Auvinen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; 16S sequencing; RNA sequencing; spatially resolved transcriptomics; antibody-based measurements; Bioteknologi; Biotechnology;

    Sammanfattning : Sequencing technologies and applications have pushed the limits and enabled novel studies of biological mechanisms, evolutionary relationships and communication networks between cells. The technical developments leading to single cell RNA-sequencing have enabled detection of rare cell populations while spatial resolution added insights into larger biological environments, like tissues and organs. LÄS MER

  4. 3. Spatial mapping of bacteria and transcriptomes

    Författare :Britta Lötstedt; Joakim Lundeberg; Anders F. Andersson; Petri Auvinen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; spatial transcriptomics; 16S sequencing; antibody-based measurements; spatiell transkriptomik; 16S-sekvensering; antikroppsbaserade mätningar; Biotechnology; Bioteknologi;

    Sammanfattning : Novel insights into biological functions and mechanisms, cell networks and evolutionary relationships are gained through development of sequencing technologies and sequencing based applications. Massively parallel sequencing has enabled analysis of big data at gene and protein expression levels, but has also characterized bacterial communities. LÄS MER

  5. 4. Global Proteome Survey -Transforming antibody-based affinity proteomics into a global discovery platform

    Författare :Niclas Olsson; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; affinity proteomics; recombinant antibodies; Proteomics; mass spectrometry; proteome profiling; breast cancer; antibody specificity;

    Sammanfattning : Proteomics is expected to generate new insights into biological processes as well as identify novel biomarkers and therapeutic targets since most biological functions are transmitted through proteins. However, due to the complexity displayed by a proteome and inherent limitations associated with current methodologies, proteomic analyses often result in incomplete coverage and inconsistent measurements. LÄS MER