Sökning: "antibody validation"

Visar resultat 6 - 10 av 34 avhandlingar innehållade orden antibody validation.

1. 6. Validation of antibodies for protein profiling : A study using immunohistochemistry on tissue microarrays

   Författare :Linda Paavilainen; Fredrik Pontén; Ola Nilsson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; antibody validation; monospecific antibodies; protein profiling; immunohistochemistry; tissue microarrays; western blot; fixatives; Pathology; Patologi; Pathology; patologi;

   Sammanfattning : The field of proteomics has rapidly expanded due to the completion of the human genome sequence. This thesis validates affinity-purified monospecific antibodies of polyclonal origin, for protein profiling in a broad spectrum of normal tissues and cells. LÄS MER

3. 7. Validation of antibodies for tissue based immunoassays

   Författare :Sandra Andersson; Anna Asplund; Fredrik Pontén; Kenneth Wester; Mike Taussig; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody validation; Conjugation; Estrogen receptor-beta; IHC; ISH; Lymphohematopoietic tissues; Proteomic; RNAseq; TMA; Transcriptomic; Patologi; Pathology;

   Sammanfattning : In situ protein detection in human tissues using antibodies reveals the cellular protein localization, and affinity-based proteomic studies can help to discover proteins involved in the development of diseases. However, antibodies often suffer from cross-reactivity, and the lack of positive and negative tissue controls for uncharacterized proteins complicates the mapping of the proteome. LÄS MER

4. 8. Antibody-based subcellular localization of the human proteome

   Författare :Marie Skogs; Emma Lundberg; Hans Blom; Cecilia Williams; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Human proteome; Subcellular localization; Organelles; Immunofluorescence; Fixation; Permeabilization; Antibody validation; Bioteknologi; Biotechnology;

   Sammanfattning : This thesis describes the use of antibodies and immunofluorescence for subcellular localization of proteins. The key objective is the creation of an open-source atlas with information on the subcellular location of every human protein. LÄS MER

5. 9. On the application and validation of multiplexed affinity assays

   Författare :Tea Dodig-Crnković; Jochen M. Schwenk; Mun-Gwan Hong; Peter Nilsson; Norman Leigh Anderson; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Affinity proteomics; Antibody; Autoantibody; Multiplexed assays; Protein microarray; Plasma proteins; Suspension bead array; Bioteknologi; Biotechnology;

   Sammanfattning : Proteins are essential macromolecules that carry out complex functions in human cells, tissues, and organs. They regulate a diverse set of biological processes and protect against pathogens. However, dysregulation or malformation of proteins can cause disease. LÄS MER

7. 10. Strategies for Miniaturized Biomarker Detection

   Författare :Belinda Adler; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biomarker; miniturization; lab-on-a-chip; proteomics; PSA; microarray; mass spectrometry; MALDI MS; SPE; sample preparation; immunoaffinity; antibody; aptamer; mass tag; ISET.;

   Sammanfattning : The aim of this thesis is development and application of different miniaturized strategies for detection of biomarkers. The biomarker PSA (prostate specific antigen), which is prostate cancer specific, has been the main focus of the thesis. LÄS MER