Sökning: "antibody structure and function"

Visar resultat 1 - 5 av 32 avhandlingar innehållade orden antibody structure and function.

  1. 1. Sequence- and structure guided engineering of proteins and enzymes for biotechnology and health applications

    Författare :Karen Schriever; Per-Olof Syrén; Elton P. Hudson; Eric A. Gaucher; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein engineering; enzymology; ancestral sequence reconstruction; antigen engineering; structural biology; terpene cyclases; enzyme thermodynamics; biocatalysis; phosphatases; allosteric regulation; Biotechnology; Bioteknologi;

    Sammanfattning : Proteins are highly diverse and sophisticated biomolecules that represent a cornerstone of biological structure and function and have been exploited in man-made applications for thousands of years. Those proteins that facilitate chemical reactions at physiologically relevant time-scales are referred to as enzymes. LÄS MER

  3. 2. Molecular Pharmacology and Structure Function Modelling of the Leukotriene B4 Receptor BLT1

    Författare :Alan Sabirsh; Institutionen för experimentell medicinsk vetenskap; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; pharmacognosy; pharmacy; toxicology; Farmakologi; farmakognosi; farmaci; toxikologi; Pharmacological sciences; fluidity; redox; chemotaxis; luciferase; calcium; ligand binding; point mutation; modelling; antibody; BLT1; leukotriene; pharmacology; GPCR; G-protein; receptor;

    Sammanfattning : Leukotrienes are membrane derived bioactive lipids that play an important role in immune responses by initiating and maintaining the inflammatory responses. Leukotriene B4 (LTB4) released at the site of an inflammatory response attracts, activates and prolongs the life of leukocytes and lymphocytes. LÄS MER

  4. 3. Methods for elucidating membrane protein structure and function

    Författare :Carolina Trkulja; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; patch clamp; antibodies; mass spectrometry; ion channels; proteomics; fluorescence; TRPV1; cholesterol.; limited proteolysis; Membrane proteins;

    Sammanfattning : Elucidation of the structure-function relationship of membrane proteins increases our understanding of many biological processes, both physiological and pathological. However, membrane proteins have been notoriously difficult to study, having part of their structure embedded into the lipid cell membrane. LÄS MER

  5. 4. Roles of unconventional ion channels and insulin granule structure in the pathogenesis of type-2 diabetes

    Författare :Mohammad Barghouth; Diabetes - öpatofysiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; T2D; pancreatic islets; mechanosensitive channels; Piezo1; beta-cell specific Piezo1 knockout mouse; KATP channel; dSTORM; Piezo1; Cav3.2; AQP1; SD; RRP; Insulin secretion and action;

    Sammanfattning : T2D is the most widespread endocrine disease. In conventional stimulus secretion coupling increased blood glucose is metabolized causing an increased intracellular level of ATP and closure of the KATP channels, and this, in turn, depolarizes the cell membrane leading to the opening of voltage-gated Ca2+ channels, the influx of Ca2+ and exocytosis of insulin granules. LÄS MER

  7. 5. Structure and Function of HAMLET: Epitopes, Membrane Interactions and Molecular Recognition

    Författare :Chin Shing Ho; immunologi och glykobiologi - MIG Avdelningen för mikrobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Sammanfattning : HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) is a complex of partially unfolded human alpha-lactalbumin and oleic acid that kills many different types of tumor cells and shows therapeutic efficacy in animal models and clinical studies. This thesis aims to (1) elucidate the structure of HAMLET and the exposure of biologically active domains, (2) define the contribution of lipids to the tumoricidal effect of HAMLET, (3) characterize the membranes response to HAMLET and the perturbation of membrane associated signaling cascades, (4) use proteomic screens to identify conserved features of HAMLET targets in tumor cells. LÄS MER