Sökning: "antibody labeling"

Visar resultat 6 - 10 av 22 avhandlingar innehållade orden antibody labeling.

1. 6. Radiolabeling and Biotinylation of Internalizing Monoclonal Antibody Chimeric BR96: Potential Use for Extracorporeal Immunoadsorption

   Författare :Jianqing Chen; Lund Medicinsk strålningsfysik; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; serologi; Immunologi; transplantation; Immunology; serology;

   Sammanfattning : In this thesis, methodology of radiolabeling and simultaneous biotinylation for internalizing monoclonal antibody (MAb) chimeric BR96 (chiBR96) have been investigated by using three element groups of potential therapeutic radionuclides iodine, indium and rhenium, and their different labeling methods. The biodistribution and kinetics of biotinylated and radiolabeled chiBR96 have been studied in colon carcinoma isografted rats. LÄS MER

3. 7. Methods for structural studies of an antibody, screening metabolites in rat urine and analysis of spent cell cultivation media using LC/ESI-MS and chemometrics

   Författare :Leila Zamani; Sven P. Jacobsson; Jonas Bergqvist; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Screening; Fingerprinting; metabolomics; Protein drugs; Mammalian Cell lines; LC ESI-MS; chemometrics; Analytical chemistry; Analytisk kemi; Analytical Chemistry; analytisk kemi;

   Sammanfattning : This thesis describes bioanalytical methods for generating fingerprints of biological systems for extracting relevant information with (protein) drugs in focus. Similarities and differences between samples can reveal the hidden relevant information, which can be used to optimize the production and facilitate the quality control of such protein drugs during their development and manufacture. LÄS MER

4. 8. Development of Flourescence-based Immunosensors for Continous Carbohydrate Monotoring : Applications for Maltose and Glucose

   Författare :Henrik Engström; Sten Ohlson; Per Ola Andersson; Christopher Lowe; Högskolan i Kalmar; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Carbohydrate; Diabetes; Immunosensor; ; Glucose; Maltose; Weak affinity chromatograhpy; Kolhydrater; Glukos; Maltose; Affinitetskromatografi; Diabetes; Biochemistry; Biokemi; Bioteknik; Biotechnology;

   Sammanfattning : Weak affinity interaction of monoclonal antibodies and carbohydrate antigens can be detected and quantified by alterations in the antibodies' intrinsic tryptophan fluorescence. These weak/transient binding events have been monitored by total internal reflection fluorescence (TlRF) by facilitating the change in intrinsic tryptophan fluorescence. LÄS MER

5. 9. Poplar wood formation : genotypical influences on structure and chemistry

   Författare :David Sandquist; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
   Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES;

   Sammanfattning : Genotypical effects on wood formation in aspen (Populus tremula x tremuloides) have been studied at morphological, ultrastructural and micro-distributional levels. To characterize transgenically in­duced modifications, light-, fluorescence-, UV-, scanning- and transmission electron microscopy have been applied complement­ed by immunolabeling, histochemistry, multivariate statistics and image analysis. LÄS MER

7. 10. Neuronal tracers for fine caliber spinal primary afferents and their response to peripheral nerve injury

   Författare :H Fredrik Wang; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Rat; Primary afferents; Dorsal root ganglion; Peripheral nerve injury; Spinal cord; Isolectin GSA I-B4; Soybean agglutinin; Cholera toxin B subunit; Horseradish peroxidase; Immunohistochemistry; Neuronal tracing;

   Sammanfattning : The isolectin B4 from Griffonia simplicifolia I (B4) and soybean agglutinin from the plant Glycine max (SBA), have been shown to bind to rat small-diameter dorsal root ganglion (DRG) neurons, and to fibers and presumed terminals in laminae I-II of the spinal cord dorsal horn. In the present thesis, these lectins and/or their horseradish peroxidase (HRP) conjugates, have been investigated in the rat with regard to their usefulness as selective neuronal markers of and axonally transported tracers for somatic and/or visceral spinal primary afferents with fine caliber, including unmyelinated, axons. LÄS MER