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Visar resultat 1 - 5 av 22 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Development of a covalent site-specific antibody labeling strategy by the use of photoactivable Z domains

   Författare :Anna Konrad; Sophia Hober; Hjalmar Brismar; Patrik Samuelsson; KTH; []
   Nyckelord :antibody labeling; site-specific; covalent; SPPS; Z domain; BPA; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : The joining of two molecular functions or the strategy of adding functions to proteins has been tremendously important for the development of proteins as tools in research and clinic. Depending on the intended application, there are a wide variety of functions that can be added to a proteins. LÄS MER

3. 2. Barcoded DNA Sequencing for Parallel Protein Detection

   Författare :Mahya Dezfouli; Afshin Ahmadian; Ulf Landegren; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA barcoding; antibody labeling; antibody oligonucleotide bio-conjugation; DNAassisted proteomics; immuno-sequencing I-Seq ; droplet-based system; large-scale data analysis; Biotechnology; Bioteknologi;

   Sammanfattning : The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. LÄS MER

4. 3. Bioimaging for analysis of protein expression in cells and tissues using affinity reagents

   Författare :Emma Lundberg; Torbjörn Gräslund; Mathias Uhlén; Rainer Pepperkok; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Affibody; antibody; biomarker; cell line; cell microarray; colorectal cancer; confocal microscopy; HER2; immunohistochemistry; immunofluorescence; light microscopy; protein expression; protein localization; SATB2; tissue microarray; Bioengineering; Bioteknik; Cell and molecular biology; Cell- och molekylärbiologi;

   Sammanfattning : The detection and analysis of biomolecules, such as proteins, are of great interest since these molecules are fundamental for life and our health. Due to the complexity of biological processes, there is a great advantage of studying proteins in their natural context, for example by using bioimaging. LÄS MER

5. 4. Site-specific labeling of affinity molecules for in vitro and in vivo studies

   Författare :Anna Perols; Amelie Eriksson Karlström; Christian Hackenberger; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; molecular imaging; site-specific labeling; solid phase peptide synthesis; IgG-binding domains; photoconjugation.; Bioteknologi; Biotechnology;

   Sammanfattning : The thesis is focused on site-specific labeling of affinity molecules for different applications where two types of binding proteins, Affibody molecules and antibodies, have been used. For the purpose of improving the properties of Affibody molecules for in vivo imaging, novel bi-functional chelators for radiolabeling using the radionuclide 111In were evaluated. LÄS MER

7. 5. Validation of antibodies for tissue based immunoassays

   Författare :Sandra Andersson; Anna Asplund; Fredrik Pontén; Kenneth Wester; Mike Taussig; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody validation; Conjugation; Estrogen receptor-beta; IHC; ISH; Lymphohematopoietic tissues; Proteomic; RNAseq; TMA; Transcriptomic; Patologi; Pathology;

   Sammanfattning : In situ protein detection in human tissues using antibodies reveals the cellular protein localization, and affinity-based proteomic studies can help to discover proteins involved in the development of diseases. However, antibodies often suffer from cross-reactivity, and the lack of positive and negative tissue controls for uncharacterized proteins complicates the mapping of the proteome. LÄS MER