Sökning: "amplicon"

Visar resultat 1 - 5 av 48 avhandlingar innehållade ordet amplicon.

1. 1. Culture-independent methods and high-throughput sequencing applied to evolutionary microbial genomics

   Författare :Anders E. Lind; Thijs JG Ettema; Purificación López-García; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Single cell genomics; metagenomics; amplicon; archaea; haloarchaea; endosymbiosis; ciliate; hydrogenosome; tree reconciliation;

   Sammanfattning : Culture-independent methods allow us to better understand the diversity of microorganisms on earth. By omitting the culturing step, we can gain access to species previously out of our reach.To better capture and understand the diversity of microorganisms, we have developed an alternative to 16S amplicon sequencing. LÄS MER

3. 2. Tagging systems for sequencing large cohorts

   Författare :Mårten Neiman; Afshin Ahmadian; Peter Savolainen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; next generation sequencing; genotyping; massive parallel sequencing; 454; Pyrosequencing; amplicon sequencing; enrichment; DNA barcodes; Genetics; Genetik;

   Sammanfattning : Advances in sequencing technologies constantly improves the throughput andaccuracy of sequencing instruments. Together with this development comes newdemands and opportunities to fully take advantage of the massive amounts of dataproduced within a sequence run. LÄS MER

4. 3. Exploration of bacteria associated with Anopheles mosquitoes around the world : For the prevention of transmission of malaria

   Författare :Louise K. J. Nilsson; Olle Terenius; Staffan Svärd; Sebastian Håkansson; Michael Strand; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; microbiota; microbiome; vector-borne disease; 16S rRNA gene; amplicon sequencing; Biologi med inriktning mot mikrobiologi; Biology with specialization in Microbiology;

   Sammanfattning : Every year, hundreds of thousands of people die from malaria. Malaria is a disease caused by parasites, which are spread by female vector mosquitoes of the genus Anopheles. Current control measures against malaria are based on drugs against the parasites and vector control using insecticides. LÄS MER

5. 4. Microbial biofilm communities associated with degradation of sprayed concrete in subsea tunnels

   Författare :Sabina Karacic; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; marine environment; subsea tunnels; surface structure; biofilm; steel fibers; metagenomics.; microbially induced deterioration; amplicon sequencing; concrete;

   Sammanfattning : Deterioration of concrete leads to reduced structural strength implying high societal challenge with huge economic impact. In the Oslofjord subsea tunnel, complex microbial biofilm activity together with abiotic attack from saline groundwater are responsible for concrete matrix degradation and steel fiber corrosion. LÄS MER

7. 5. MHC diversity and expression in a songbird : Temporal and transgenerational variation

   Författare :Samantha Mellinger; MEMEG; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MHC diversity; MHC-I gene expression; songbirds; genome assembly; amplicon HTS; temporal variation; family data; great reed warbler Acrocephalus arundinaceus ;

   Sammanfattning : The immune system is of central importance to clear-off pathogens in order to keep an organism healthy. In vertebrates, the Major Histocompatibility Complex (MHC) is a genomic region that holds key genes involved in adaptive immunity and in particular in the antigen presenting process. LÄS MER