Sökning: "amino acid mutagenesis"

Visar resultat 1 - 5 av 78 avhandlingar innehållade orden amino acid mutagenesis.

  1. 1. Mutational Analysis and Redesign of Alpha-class Glutathione Transferases for Enhanced Azathioprine Activity

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Olof Modén; Uppsala universitet.; [2013]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; allelic variants; azathioprine; bioactivation; chimeric mutagenesis; directed evolution; DNA shuffling; enzyme engineering; glutathione transferase; GST; lysate screening; molecular docking; multiple alignment; multivariate analysis; polymorphism; principal component analysis; prodrug; prodrug activation; protein engineering; protein redesign; reduced amino acid alphabet; saturation mutagenesis; semi-rational enzyme engineering; site-directed mutagenesis; structure-activity relationship; structure-based redesign; Biokemi; Biochemistry;

    Sammanfattning : Glutathione transferase (GST) A2-2 is the human enzyme most efficient in catalyzing azathioprine activation. Structure-function relationships were sought explaining the higher catalytic efficiency compared to other alpha class GSTs. LÄS MER

  2. 2. Expression of Manganese Lipoxygenase and Site-Directed Mutagenesis of Catalytically Important Amino Acids Studies on Fatty Acid Dioxygenases

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Mirela Cristea; Uppsala universitet.; [2006]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical pharmacology; Dioxygenase; Gaeumannomyces graminis; lipoxygenase; Magnaporthe grisea; oxylipin; Pichia pastoris; hydroperoxide isomerase; polyunsaturated fatty acids; Farmaceutisk farmakologi; PHARMACY Pharmaceutical pharmacology; FARMACI Farmaceutisk farmakologi;

    Sammanfattning : Polyunsaturated fatty acids can be bioactivated by two families of dioxygenases, which either contain non-heme iron (lipoxygenases) or heme (cyclooxygenases, linoleate diol synthases and α-dioxygenases).Lipoxygenases and their products play important roles in the pathophysiology of plants and fungi. LÄS MER

  3. 3. Characterization of galanin receptors using chimeric peptides and site-directed mutagenesis

    Detta är en avhandling från Stockholm : Department of Neurochemistry, Stockholm University

    Författare :Ulla Eriksson Sollenberg; Stockholms universitet.; [2010]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; galanin; GPCR; mutagenesis; neuropeptide; MEDICINE Chemistry Neurochemistry; MEDICIN Kemi Neurokemi; Neurochemistry and Neurotoxicology; neurokemi och neurotoxikologi;

    Sammanfattning : Galanin, a 29 (30) amino acid neuropeptide, is found throughout both the central and peripheral nervous systems. It signals via three receptors, GalR1-3, all belonging to the rhodopsin-like G-protein coupled receptors. Galanin and its receptors have been implicated in a vast variety of biological processes. LÄS MER

  4. 4. Receptor-ligand interactions of the human 5-HT1B receptor : A site-directed mutagenesis study

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Charlotta Grånäs; Uppsala universitet.; [1998]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Neurosciences; 5-hydroxytryptamine; serotonin; 5-HT1B receptor; site-directed mutagenesis; signal transduction; sumatriptan; G protein; Neurovetenskap; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Internal medicine Neurology; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Invärtesmedicin Neurologi; medicinsk farmakologi; Medical Pharmacology;

    Sammanfattning : The 5-hydroxytryptamine (5-HT, serotonin)1B receptor is one of thirteen G protein-coupled 5-HT receptors. 5-HT is known to influence temperature regulation, pain, cognition and sexual and feeding behaviours. LÄS MER

  5. 5. Exotic Ribosomal Enzymology

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Josefine Liljeruhm; Uppsala universitet.; [2019]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; rRNA modification; Methyltransferase; RlmM; RlmJ; D-amino acid; Unnatural amino acid; Chromoprotein; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Sammanfattning : This thesis clarifies intriguing enzymology of the ribosome, the multiRNA/multiprotein complex that catalyzes protein synthesis (translation). The large ribosomal RNAs (23S and 16S rRNAs in E. coli) are post-transcriptionally modified by many specific modification enzymes, yet the functions of the modifications remain enigmatic. LÄS MER