Sökning: "amino acid exchange"

Visar resultat 1 - 5 av 33 avhandlingar innehållade orden amino acid exchange.

1. 1. Physiology of Escherichia coli in batch and fed-batch cultures with special emphasis on amino acid and glucose metabolism

   Författare :Ling Han; KTH; []
   Nyckelord :escherichia coli; amino acids; glycine; quorum sensing; metabolic switch; metabolite po9ols; carbon balance; outer membrne; lipopolysaccharide; batch culture;

   Sammanfattning : The objective of this work is to better understand themetabolism and physiology ofEscherichiacoli(W3110) in defined medium cultures with thelong-term goal of improving cell yield and recombinant proteinproductivity.The order of amino acid utilization inE. colibatch cultures was investigated in a medium with16 amino acids and glucose. LÄS MER

3. 2. Protein Engineering on Azurin. Expression Mutagenesis and Characterisation of Copper Site Mutants

   Författare :B Göran Karlsson; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; EPR spectroscopy; cassette mutagenesis; optical spectroscopy; amino acid exchange; Escherichia coli; electron transfer; reduction; azurin;

   Sammanfattning : Azurin belongs to a family of small blue copper proteins or cupredoxins which participate in electron transfer processes in plants and bacteria. The type 1 copper site in these proteins is characterised by an intense blue colour, a narrow hyperfine coupling in the EPR signal and, generally, a high reduction potential. LÄS MER

4. 3. Advances in Ligand Binding Predictions using Molecular Dynamics Simulations

   Författare :Henrik Keränen; Johan Åqvist; Jordi Villà i Freixa; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; free-energy perturbation; molecular dynamics; ligand binding; free-energy perturbation; linear interaction energy; binding free-energy; homology modeling; virtual screening; alanine scanning; amino acid mutagenesis; hERG; GPCR; adenosine receptor; serotonin receptor; BRICHOS; cruzain;

   Sammanfattning : Biochemical processes all involve associations and dissociations of chemical entities. Understanding these is of substantial importance for many modern pharmaceutical applications. In this thesis, longstanding problems with regard to ligand binding are treated with computational methods, applied to proteins of key pharmaceutical importance. LÄS MER

5. 4. Asymmetric [2,3]-Sigmatropic Rearrangement of Allylic Ammonium Ylides

   Författare :Jan Blid; Peter Somfai; James C. Anderson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; asymmetric; [2; 3]-sigmatropic rearrangement; allylic amine; ammonium ylide; Lewis acid; enantioselective; boron; phosphazene base; NMR spectroscopy; DFT-calculations; Organic chemistry; Organisk kemi;

   Sammanfattning : The thesis describes the realization of an asymmetric [2,3]-sigmatropic rearrangement of achiral allylic amines. It is divided into two parts; the first part deals with the development of a Lewis acid-mediated [2,3]-sigmatropic rearrangement and the second the asymmetric version thereof. LÄS MER

7. 5. The Folding Energy Landscape of MerP

   Författare :Ann-Christin Brorsson; Bengt-Harald Jonsson; Daniel Otzen; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; protein folding and stability; hydrogen exchange; intermediate; partial unfolding; Biokemi; Biochemistry; Biokemi; Biochemistry; biokemi;

   Sammanfattning : This thesis is based on studies, described in four papers, in which the folding energy landscape of MerP was investigated by various techniques. MerP is a water-soluble 72 amino acid protein with a secondary structure consisting of four anti-parallel β-strands and two α-helices on one side of the sheet in the order β1α1β2β3α2β4. LÄS MER