Sökning: "amino acid and peptide separation"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden amino acid and peptide separation.

  1. 1. Sensitivity and selectivity studies in capillary electrophoresis

    Författare :Maria Petersson; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; peptides; dansyl amino acids; imaging detection; cyclodextrins; enantiomer separation; acoustic levitation; solid-phase extraction; preconcentration; capillary electrophoresis; micellar electrokinetic chromatography; drugs; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Capillary electrophoresis is characterised by high separation efficiency, applicability to a wide range of compounds, small sample and solvent consumption and simple instrumentation. To improve sensitivity using on-line absorbance detectors, which are hampered by the short optical path length, sample volumes in the microlitre range were concentrated to nanolitre volumes either off-line or on-line. LÄS MER

  3. 2. Mass spectrometry in peptide and tumor marker analysis

    Författare :Ann-Charlotte Bergman; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Integration of preparative protein and peptide separations with analytical techniques, in particular mass spectrometry (MS), is important. This thesis describes preparation via capillary electrophoresis and two-dimensional (2-D) gel electrophoresis for identification and structure determination of polypeptides. LÄS MER

  4. 3. Partitioning of biomolecules in thermoseparating polymer-water two-phase systems

    Författare :Hans-Olof Johansson; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aqueous two-phase system; temperature-induced phase separation; amino acid and peptide separation; polymer; partitioning; Biology; Biologi;

    Sammanfattning : The partitioning behaviour of different types of molecules in thermoseparating two-phase systems has been studied. A thermoseparating two phase system was formed by heating a water solution, which contained a thermoseparating polymer, above the cloud point temperature. LÄS MER

  5. 4. Isolation and characterization of lipid-associated and neurosecretory polypeptides

    Författare :Margareta Stark; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Lipid-interacting proteins play important roles in all living organisms. This thesis focuses on isolation and characterization of an enzyme in the triacylglycerol biosynthesis (phosphatidic acid phosphatase, PAP), hydrophobic polypeptides in bile, and polypeptides in cerebrospinal fluid. LÄS MER

  7. 5. Multimodal Chemical Imaging of Amyloid Plaque Pathology in Alzheimer’s Disease

    Författare :Wojciech Michno; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Alzheimer’s disease; beta-amyloid; electron microscopy; imaging mass spectrometry; lipids; luminescent conjugated oligothiophenes; matrix-assisted laser desorption ionization mass spectrometry MALDI-MS ; molecular imaging; nanoscale secondary ion mass spectrometry NanoSIMS ; neurodegeneration; peptides;

    Sammanfattning : Alzheimer’s disease (AD) is the most common form of dementia. AD has been linked to the aggregation of amyloid beta (Aβ) peptides into extracellular deposits, Aβ plaques. These are also found in cognitively unimpaired amyloid-positive (CU-AP) individuals, but these Aβ plaques are primarily diffuse in structure. LÄS MER