Sökning: "albumin-binding domain ABD"

Visar resultat 1 - 5 av 19 avhandlingar innehållade orden albumin-binding domain ABD.

  1. 1. Protein engineering to explore and improve affinity ligands

    Författare :Martin Linhult; KTH; []
    Nyckelord :binding; affinity; human serum albumin HSA ; albumin-binding domain ABD ; affinity chromatography; deamidation; protein A; stabilization; Z-domain; capacity; protein G; cleaning-in-place CIP ; protein engineering; C2 receptor;

    Sammanfattning : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. LÄS MER

  2. 2. Chemical Engineering of Small Affinity Proteins

    Författare :Joel Lindgren; Amelie Eriksson Karlström; Lars Baltzer; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; albumin binding domain; ligation; protein synthesis; solid phase peptide synthesis; Bioteknologi; Biotechnology;

    Sammanfattning : Small robust affinity proteins have shown great potential for use in therapy, in vivo diagnostics, and various biotechnological applications. However, the affinity proteins often need to be modified or functionalized to be successful in many of these applications. LÄS MER

  3. 3. Development of molecular recognition by rational and combinatorial engineering

    Författare :Andreas Jonsson; Stefan Ståhl; Mats Persson; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Affibody molecule; albumin binding domain; protein engineering; phage display; amyloid beta peptide; TNF; HSA; Molecular biology; Molekylärbiologi;

    Sammanfattning : Combinatorial protein engineering, taking advantage of large libraries of protein variants and powerful selection technology, is a useful strategy for developing affinity proteins for applications in biotechnology and medicine. In this thesis, two small affinity proteins have been subjected to combinatorial protein engineering to improve or redirect the binding. LÄS MER

  4. 4. Exploitation of interactions with the neonatal Fc receptor to manipulate biological half-lives for therapeutic applications

    Författare :Shengze Yu; Torbjörn Gräslund; Mats Ohlin; KTH; []
    Nyckelord :Glioblastoma Multiforme GBM ; Prolacin Prl ; Prolacin receptor PrlR ; Prolactin antagonist PrlRA ; Albumin binding domain ABD ; serum albumin SA ; half-life extension; FcRn; Affibody; human growth hormone hGH ; immunoglobulin G IgG ; blocking; autoimmune disease; Bioteknologi; Biotechnology;

    Sammanfattning : Protein engineering provides powerful tools to create useful proteins with desired properties. In this thesis, rational design principles have been used for development of fusion proteins that can interact with the neonatal Fc receptor (FcRn) for potential medical applications. FcRn is widely expressed in the human body. LÄS MER

  5. 5. Development of ADAPT-based tracers for radionuclide molecular imaging of cancer

    Författare :Javad Garousi; Vladimir Tolmachev; Tony Lahoutte; Uppsala universitet; []
    Nyckelord :ADAPT; scaffold protein; albumin-binding domain ABD ; Affinity protein; HER2; radionuclide molecular imaging;

    Sammanfattning : ABD-Derived Affinity Proteins (ADAPTs) is a novel class of small engineered scaffold proteins based on albumin-binding domain (ABD) of streptococcal protein G. High affinity ADAPT  binders against various therapeutic targets can be selected. LÄS MER