Sökning: "affinity enhancement"

Visar resultat 1 - 5 av 27 avhandlingar innehållade orden affinity enhancement.

  1. 1. Exploring molecular interactions between polypeptide conjugates and protein targets : Manipulating affinity by chemical modifications

    Författare :Aleksandra Balliu; Lars Baltzer; Oliver Seitz; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; polypeptide conjugates; protein targets; molecular recognition; affinity enhancement; conjugation;

    Sammanfattning : In this thesis molecular interactions between polypeptide conjugates and protein targets were investigated. Polypeptides were derivatized with small organic molecules, peptides and oligonucleotides. New strategies were developed with the aim to increase affinities for proteins of biological interest. LÄS MER

  2. 2. Fc receptors and feedback enhancement of antibody responses

    Författare :Sara Wernersson; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; mouse; transgenic knockout; immune regulation; B lymphocytes; Fc receptors; IgG; IgE; FcgRI; FcgRII; FcgRIII; CD23; Genetik; Clinical genetics; Klinisk genetik; Pathology; patologi;

    Sammanfattning : Antibodies are important for the defence against microorganisms, but are also involved inpathological reactions such as allergy or autoimmunity. The exact mechanisms involved inregulation of antibody responses is still largely unknown. In this thesis, immune regulationby antibodies and the receptors they bind to (FcR), were investigated. LÄS MER

  3. 3. Elicitation and enhancement of T and B cell responses

    Författare :Hannes Uchtenhagen; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP;

    Sammanfattning : The Major histocompatibility complex class I (MHC-I) has been characterized in such great depth that a number of its key properties are well understood and part of its behavior can even be predicted. It is therefore intriguing that the impact of small epitope modifications on immunogenicity and the elicitation of T-cell repertoires remain often unpredictable and full of surprises. LÄS MER

  4. 4. Feedback Enhancement of Antibody Responses via Complement and Fc Receptors

    Författare :Jörgen Dahlström; Uppsala universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Mouse; transgenic knockout; immune regulation; B lymphocytes; Fc receptors; complement; IgG; IgM; IgE; Fc-gamma-RI; Fc-gamma-RII; Fc-gamma-RIII; CR1; CR2; CD21; CD35; Genetik; Clinical genetics; Klinisk genetik; Pathology; patologi;

    Sammanfattning : IgG, IgM and IgE in complex with antigen have the capacity to regulate specific immune responses. In this investigation, the role of Fc receptors for IgG (FcγRI, FcγRII and FcγRIII) and complement receptors 1 and 2 (CR1/2) for antibody-mediated enhancement of antibody responses are investigated. LÄS MER

  5. 5. Conformationally Constrained Nucleosides, Nucleotides and Oligonucleotides : Design, Synthesis and Properties

    Författare :Dmytro Honcharenko; Jyoti Chattopadhyaya; Piet Herdewijn; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; antisense oligonucleotides; conformationally constrained nucleosides; azetidine; aza-ENA; target affinity; RNase H; exonuclease stability; pyrene-functionalized nucleotides; fluorescence; mismatch discrimination; Bioorganisk kemi; Cell and molecular biology; Cell- och molekylärbiologi;

    Sammanfattning : This thesis is based on six original research publications describing synthesis, structure and physicochemical and biochemical analysis of chemically modified oligonucleotides (ONs) in terms of their potential diagnostic and therapeutic applications. Synthesis of two types of bicyclic conformationally constrained nucleosides, North-East locked 1',2'-azetidine and North locked 2',4'-aza-ENA, is described. LÄS MER