Sökning: "affinity domain"

Visar resultat 1 - 5 av 211 avhandlingar innehållade orden affinity domain.

1. 1. Protein engineering to explore and improve affinity ligands

   Författare :Martin Linhult; KTH; []
   Nyckelord :binding; affinity; human serum albumin HSA ; albumin-binding domain ABD ; affinity chromatography; deamidation; protein A; stabilization; Z-domain; capacity; protein G; cleaning-in-place CIP ; protein engineering; C2 receptor;

   Sammanfattning : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. LÄS MER

3. 2. An albumin-binding domain as a scaffold for bispecific affinity proteins

   Författare :Johan Nilvebrant; Sophia Hober; Andrew Bradbury; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; albumin-binding domain; bispecific; albumin; affinity protein; phage display; staphylococcal display; orthogonal affinity purification; TNF-alpha; ErbB2; ErbB3; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : Protein engineering and in vitro selection systems are powerful methods to generate binding proteins. In nature, antibodies are the primary affinity proteins and their usefulness has led to a widespread use both in basic and applied research. LÄS MER

4. 3. Protein microarrays for validation of affinity binders

   Författare :Mårten Sundberg; Peter Nilsson; Jochen Schwenk; Anders Isaksson; Afshin Ahmadian; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microarray; protein; antibody; antigen; affinity; validation;

   Sammanfattning : Is specificity an important issue regarding affinity reagents? What about the validation of affinity reagents today, is it good enough? This depends on the application and the producer of the reagent. Validation should be the most important marketing argument that can be found. LÄS MER

5. 4. Chemical Engineering of Small Affinity Proteins

   Författare :Joel Lindgren; Amelie Eriksson Karlström; Lars Baltzer; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; albumin binding domain; ligation; protein synthesis; solid phase peptide synthesis; Bioteknologi; Biotechnology;

   Sammanfattning : Small robust affinity proteins have shown great potential for use in therapy, in vivo diagnostics, and various biotechnological applications. However, the affinity proteins often need to be modified or functionalized to be successful in many of these applications. LÄS MER

7. 5. Investigations of chemical and enzymatic functionalization of affinity proteins

   Författare :Anders Myrhammar; Amelie Eriksson Karlström; Per-Åke Nygren; Christian Heinis; KTH; []
   Nyckelord :Affinity protein; affibody; conjugation; solid phase peptide synthesis; proteolytic stability; peptide nucleic acid PNA .; Bioteknologi; Biotechnology;

   Sammanfattning : AbstractAffinity proteins are important reagents in research, diagnostics and therapeutic settings. The focus of this thesis has been on investigating different chemical and enzymatic strategies for engineering of affinity proteins to generate affinity reagents with improved or changed functionality. LÄS MER