Sökning: "affibodies"

Visar resultat 1 - 5 av 16 avhandlingar innehållade ordet affibodies.

  1. 1. Affibodies

    Författare :Karin Nord; KTH; []
    Nyckelord :;

    Sammanfattning : The introduction of recombinant DNA technology-based methodsfor the overproduction of either wild type or engineeredproteins in various host cells has had an enormous impact onthe availability of proteins for various therapeutic,diagnostic and research applications, which also has creatednew demands on efficient production and purification methods toprovide the desired material. Affinity chromatography hasproven to be an outstanding technique to obtain a single-stepincrease in purity and concentration. LÄS MER

  3. 2. Engineering Yeast Metabolism for Production of Sesquiterpenes

    Författare :Stefan Tippmann; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Saccharomyces cerevisiae; affibodies; transcriptomics; isoprenoids; sesquiterpenes; metabolomics; metabolic engineering;

    Sammanfattning : Sesquiterpenes belong to the large and diverse group of isoprenoids, which are ubiquitous in the plant kingdom and have various applications in the chemical industry as fragrances, pharmaceuticals and as substitutes for petroleum-based gasoline, diesel and jet fuels. In this project, production of sesquiterpenes was studied in Saccharomyces cerevisiae using farnesene as an example with the objective to gain new insights into the synthesis of these compounds and to evaluate different metabolic engineering strategies. LÄS MER

  4. 3. Development of new affinity proteins for neurodegenerative disorders

    Författare :Linnea C. Hjelm; John Löfblom; Stefan Ståhl; Hanna Lindberg; Ylva Ivarsson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein engineering; sequestrins; amyloid beta; alpha-synuclein; phage display; affibodies; blood–brain barrier; Transferrin receptor; receptor-mediated transcytosis; recombinant spider silk; Biotechnology; Bioteknologi;

    Sammanfattning : Neurodegenerative disorders include a full spectrum of diagnoses, including dementias and other neuronal diseases, characterised by degradation of neurons in the brain occurring along with disease progression. Amongst the dementias, the most prevalent are Alzheimer’s (AD) and Parkinson’s disease (PD) that affect millions of people worldwide. LÄS MER

  5. 4. Display of affinity proteins on bacteria and bacteriophage

    Författare :Elin Gunneriusson; KTH; []
    Nyckelord :;

    Sammanfattning : Surface display of peptides and proteins on microorganismshas during the last decade gained tremendous interest due tothe numerous applications for the technique found inbiotechnology, molecular biology and immunology. This thesisdescribes both bacterial display of different affinity proteinsand display of libraries of small α-helical proteindomains on phage for selection of novel binding proteinsdenoted affibodies. LÄS MER

  7. 5. Investigations of chemical and enzymatic functionalization of affinity proteins

    Författare :Anders Myrhammar; Amelie Eriksson Karlström; Per-Åke Nygren; Christian Heinis; KTH; []
    Nyckelord :Affinity protein; affibody; conjugation; solid phase peptide synthesis; proteolytic stability; peptide nucleic acid PNA .; Bioteknologi; Biotechnology;

    Sammanfattning : AbstractAffinity proteins are important reagents in research, diagnostics and therapeutic settings. The focus of this thesis has been on investigating different chemical and enzymatic strategies for engineering of affinity proteins to generate affinity reagents with improved or changed functionality. LÄS MER