Sökning: "XR XDH"

Visar resultat 1 - 5 av 10 avhandlingar innehållade orden XR XDH.

  1. 1. Metabolic Engineering of Xylose-Utilising Saccharomyces cerevisiae Strains. A Closer Look at Recombinant Strains Based on the Xylose Reductase-Xylitol Dehydrogenase Pathway

    Författare :Marie Jeppsson; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi; microarrays; cofactors; XK; XDH; XR; lignocellulose; xylose; ethanol; Saccharomyces cerevisiae; yeast;

    Sammanfattning : Saccharomyces cerevisiae produces ethanol efficiently from the hexose sugars in lignocellulose hydrolysates, but it can not utilise pentose sugars such as xylose and arabinose. Stable xylose-utilising S. LÄS MER

  3. 2. Used but not Sensed - The Paradox of D-xylose Metabolism in Saccharomyces cerevisiae

    Författare :Karen Ofuji Osiro; Teknisk mikrobiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; saccharomyces cerevisiae; sugar sensing signalling; D-Xylose; XR XDH; GFP biosensor; cAMP PKA; Snf3p Rgt2p; SNF1 Mig1p; flow cytometry;

    Sammanfattning : The realization that the extraction and combustion of fossil fuels is having serious effects on the environment and the climate, together with the ever-growing need for fuels, has led to the development of the concept of the biorefinery. Biorefineries are refineries in which fossil resources, such as oil, are replaced by renewable biomaterials to produce biofuels and biochemicals. LÄS MER

  4. 3. Engineering xylose and arabinose metabolism in recombinant Saccharomyces cerevisiae

    Författare :Kaisa Karhumaa; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; virologi; bakteriologi; mycology; Mikrobiologi; bacteriology; Saccharomyces cerevisiae ; arabinose; xylose; fermentation; lignocellulose; yeast; mykologi; Biochemical technology; Biokemisk teknik; virology; Microbiology; ethanol;

    Sammanfattning : Utilization of all sugars in lignocellulose hydrolysates is a prerequisite for economically feasible bioethanol production. The yeast commonly used for industrial ethanol production, Saccharomyces cerevisiae, is naturally unable to utilize pentose sugars xylose and arabinose, which constitute a large fraction of many lignocellulosic materials. LÄS MER

  5. 4. Redox balancing in recombinant strains of Saccharomyces cerevisiae

    Författare :Mikael Anderlund; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; xylose isomerase; xylitol dehydrogenase; xylose reductase; glycerol; xylitol; xylose; E. coli; A. vinelandii; T. thermophilus; S. cerevisiae; P. stipitis; transhydrogenase.; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : In metabolically engineered Saccharomyces cerevisiae expressing Pichia stipitis XYL1 and XYL2 genes, encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, xylitol is excreted as the major product during anaerobic xylose fermentation and only low yields of ethanol are produced. This has been interpreted as a result of the dual cofactor dependence of XR and the exclusive use of NAD+ by XDH. LÄS MER

  7. 5. Genetic Traits Beneficial for Xylose Utilization by Recombinant Saccharomyces cerevisiae

    Författare :Oskar Bengtsson; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Saccharomyces cerevisiae; metabolic engineering; fermentation; xylose; ethanol; promoter; xylose reductase; cofactor; validation; transcriptome;

    Sammanfattning : Saccharomyces cerevisiae ferments hexoses in lignocellulosic hydrolysates under anaerobic conditions with high rates and ethanol yields. However, S. cerevisiae is naturally unable to utilize the pentose fraction of the hydrolysates. Xylose is the most abundant pentose sugar, and although recombinant S. LÄS MER