Sökning: "X-ray single-particle Imaging"

Visar resultat 1 - 5 av 18 avhandlingar innehållade orden X-ray single-particle Imaging.

  1. 1. Coherent Diffractive Imaging with X-ray Lasers

    Författare :Max Felix Hantke; Filipe R. N. C. Maia; Janos Hajdu; Martin Svenda; Edgar Weckert; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; coherent diffractive X-ray imaging; lensless imaging; coherent X-ray diffractive imaging; flash diffractive imaging; single particle imaging; aerosol injection; electrospray injection; substrate-free sample delivery; carboxysome; phase retrieval; X-ray diffraction software; X-ray free-electron laser; XFEL; FEL; CXI; CDI; CXDI; FXI;

    Sammanfattning : The newly emerging technology of X-ray free-electron lasers (XFELs) has the potential to revolutionise molecular imaging. XFELs generate very intense X-ray pulses and predictions suggest that they may be used for structure determination to atomic resolution even for single molecules. LÄS MER

  2. 2. Statistical processing of Flash X-ray Imaging of protein complexes

    Författare :Alberto Pietrini; Carl Nettelblad; Johan Bielecki; Janos Hajdu; Flavio Capotondi; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; X-ray; XFEL; single particle; hit-finder; statistical hit-finder; single protein; protein complex; RNA polymerase II; FEL; free-electron laser; FXI; Flash X-ray Imaging; CXI; CSPAD; x-ray imaging; COACS; Physics with specialization in Biophysics; Fysik med inriktning mot biofysik;

    Sammanfattning : Flash X-ray Imaging (FXI) at X-ray Free Electron Lasers (XFELs) is a promising technique that permits the investigation of the 3D structure of molecules without the need for crystallization, by diffracting on single individual sample particles.In the past few years, some success has been achieved by using FXI on quite large biological complexes (40 nm-1 μm in diameter size). LÄS MER

  3. 3. Imaging Living Cells with an X-ray Laser

    Författare :Gijs van der Schot; Tomas Ekeberg; Janos Hajdu; Filipe Maia; Pierre Thibault; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Coherent diffractive X-ray imaging; flash X-ray imaging; lensless imaging; single particle imaging; cyanobacteria; phasing; image classification; substrate-free sample delivery; X-ray free-electron laser; XFEL; FEL; CDXI; CDI; CXI; FXI; Chemistry with specialization in Biophysics; Kemi med inriktning mot biofysik;

    Sammanfattning : Imaging living cells at a resolution higher than the resolution of optical microscopy is a significant challenge. Fluorescence microscopy can achieve a degree of super-resolution via labeling cellular components with a fluorescent dye. LÄS MER

  4. 4. Ultrafast Coherent X-ray Diffractive Nanoimaging

    Författare :Filipe R. N. C. Maia; Janos Hajdu; David van der Spoel; Keith Nugent; Uppsala universitet; []
    Nyckelord :XFEL; Phasing; Image Reconstruction; Single Particle Imaging; Ultrafast Diffraction; X-ray diffraction; Coherent Diffractive Imaging; CXDI;

    Sammanfattning : X-ray lasers are creating unprecedented research opportunities in physics,chemistry and biology. The peak brightness of these lasers exceeds presentsynchrotrons by 1010, the coherence degeneracy parameters exceedsynchrotrons by 109, and the time resolution is 105 times better. LÄS MER

  5. 5. Structural Studies of Large dsDNA Viruses using Single Particle Methods

    Författare :Hemanth Kumar Narayana Reddy; Martin Svenda; Sarah Butcher; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; PR772; phage; PRD1; Bacteriophage; coliphage; Melbournevirus; Cedratvirus; lurbo; Pithovirus; CryoEM; Single particle imaging; Coherent; Diffractive; Imaging; Soft X-ray; Microscopy; Microscope; GDVN; High resolution; XFEL; aerosol; Injection; electrospray; gas dynamic virtual nozzle; CDI; CXI; FEL; Chemistry with specialization in Biophysics; Kemi med inriktning mot biofysik;

    Sammanfattning : Structural studies of large biological assemblies pose a unique problem due to their size, complexity and heterogeneity. Conventional methods like x-ray crystallography, NMR, etc. are limited in their ability to address these issues. To overcome some of these limitations, single particle methods were used. LÄS MER