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1. 1. Development of Enhanced Analytical Methodology for Lipid Analysis from Sampling to Detection : A Targeted Lipidomics Approach

   Författare :Giorgis Isaac; Jonas Bergquist; Karin Markides; Monica Waldebäck; William Christie; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; electrospray ionization; lipids; liquid chromatography; mass spectrometry; pesticides; phosphatidylcholine; pressurized fluid extraction; sphingomyelin; sulfatides; Analytisk kemi; Analytical chemistry; Analytisk kemi;

   Sammanfattning : This thesis covers a wide range of analytical method development for lipid analysis in complex biological samples; from sample preparation using pressurized fluid extraction (PFE) and separation with reversed phase capillary liquid chromatography (RP-LC) to detection by electrospray ionization mass spectrometry (ESI/MS) and tandem MS.The requirements for fast, reliable and selective extraction methods with minimal usage of solvents have accelerated the development of new extraction techniques. LÄS MER