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Visar resultat 1 - 5 av 9 avhandlingar som matchar ovanstående sökkriterier.

  1. 1. Regulation of productivity in Trichoplusia ni and Spodoptera frugiperda Sf9 serum-free cultures

    Författare :Karin Calles; Lena Häggström; Gunnel Dalhammar; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Insect cells; BEVS; Spodoptera frugiperda; Sf9; Trichoplusia ni; BTI-Tn-5B1-4; specific productivity; conditioned medium; conditioned medium factors; cell cycle; synchronization; G2 M; Bioteknik; Bioengineering; Bioteknik;

    Sammanfattning : The aim of this work has been to characterize the effects of conditioned medium (CM) on insect cell productivity and physiology in order to get a better understanding about the mechanisms that regulate productivity in serum-free media. Two cell lines have been investigated, Spodoptera frugiperda (Sf9) and Trichoplusia ni (T. ni, BTI-Tn-5B1-4). LÄS MER

  3. 2. Impact of autocrine factors on physiology and productivity in Trichoplusia ni serum-free cultures

    Författare :Ulrika Eriksson; Lena Häggström; Per Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Trichoplusia ni; High Five; insect cells; BEVS; serum-free medium; yeast extract; conditioned medium; autocrine regulation of proliferation; growth factors; cell cycle; metalloproteinase; specific productivity; Bioteknik; Bioengineering; Bioteknik;

    Sammanfattning : The aim of this study was to increase the understanding of the mechanisms regulating cell proliferation and recombinant protein production in serum-free cultures of Trichoplusia ni (T. ni) insect cells.Conditioned medium (CM) was shown to contain both stimulatory and inhibitory factors (CM factors) influencing cell growth. LÄS MER

  4. 3. Identification and characterisation of five innate immune genes in the cabbage looper Trichoplusia ni

    Författare :Annika Lundström; Lage Cerenius; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mikrobiologi; Microbiology;

    Sammanfattning : When insects are infected with bacteria, they respond by producing a range of different antibacterial peptides and by activating protease cascades leading to coagulation and melanisation. Cellular defence mechanisms are also involved in killing of microorganisms through phagocytosis and encapsulation. LÄS MER

  5. 4. Identification of immune genes in the insects Trichoplusia ni and Anopheles gambiae

    Författare :Daiwu Kang; Paul Brey; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Microbiology; Mikrobiologi; mikrobiologi; Microbiology;

    Sammanfattning : .... LÄS MER

  7. 5. Innate immune gene expression and function studied in insects and mammals

    Författare :Gang Liu; Ulrich Theopold; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mikrobiologi; Microbiology;

    Sammanfattning : Insects and mammals possess innate immune responses to effectively recognize and eliminate invading microorganisms. Insects, who share with mammals conserved molecules for immune defense, are good model systems for innate immunity studies. LÄS MER