Sökning: "Tillämpad Biokemi"

Visar resultat 1 - 5 av 67 avhandlingar innehållade orden Tillämpad Biokemi.

1. 1. Towards the Rational Design of Molecularly Imprinted Polymers

   Författare :Håkan S Andersson; Ann-Christin Koch-Schmidt; Ian A Nicholls; Klaus Mosbach; Richard F Venn; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Thermodynamics; Molecular Recognition; Polyvalency; Cooperativity; Electrostatic Interactions; Hydrophobic Effect; Chromatography; Frontal Analysis; HPLC; Spectroscopy; Molecularly Imprinted Polymer; Molecular Imprinting; Crown Ether; Template Polymerisation; Cyclodextrin; Biochemistry; Metabolism; Biokemi; Biotechnology; Bioteknik; molecular recognition; Biokemi;

   Sammanfattning : Molecular imprinting is a technique by which polymeric materials selective for a given target molecule can be created through a casting procedure. Functionalised monomers are added to a solution of molecular templates. LÄS MER

3. 2. Selenoproteins in the Bovine Mammary Gland. Regulation of mRNA and Protein Expression

   Författare :Katharina Bruzelius; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Livsmedelsteknik; Food and drink technology; metabolism; Biokemi; Metabolism; Biochemistry; Naturvetenskap; milk; Natural science; cancer; prolactin; insulin; hydrocortisone; retinoic acid; MAC-T; northern blot; two-dimensional electrophoresis; bovine mammary gland; selenium; selenoprotein;

   Sammanfattning : Selenium is a micronutrient that is essential for many important life processes due to the action of the specific selenoproteins containing one or more of the 21st amino acid, selenocysteine. Twenty-five selenoprotein genes have been found in the human genome but the function of many of them is not yet known. LÄS MER

4. 3. Gene fusion in protein engineering : design of novel peptides and bifunctional enzymes

   Författare :Sarah Fredriksson; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cadmium; Bifunctional enzyme; Catalytic triad; Channeling; Coenzyme recycling; Galactose dehydrogenase; ß-Galactosidase; Gene fusion; Hexapeptide; Lactate dehydrogenase; Linker region; Multifunctional enzymes; Oligomeric protein; NADH; Phage; OmpA; Biochemistry; Biokemi; metabolism; Biotechnology; Bioteknik;

   Sammanfattning : The gene fusion technique is widely used in protein engineering and in this thesis it constitutes the basis of both the construction of artificial bifunctional enzymes and the display of two novel peptides. Artificial bifunctional enzymes have been used as model systems in the studies of native multifunctional enzymes and proximity effects within these complexes. LÄS MER

5. 4. Selective Sorbents - Silica-immobilized affinity ligands and molecularly imprinted polymers

   Författare :Magnus Glad; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular imprinting; HPLAC; composites; TRIM; Biokemi; Biochemistry; Metabolism; affinity chromatography;

   Sammanfattning : The development of selective sorbents for the separation of various molecules, preferentially biomolecules, is described in this work. Affinity ligands were immobilized on small silica particles and used in high-performance liquid affinity chromatography (HPLAC). LÄS MER

7. 5. Superporous agarose a new material for chromatography

   Författare :Per-Erik Gustavsson; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein separations; Chromatography; Superporous agarose; Continuous agarose beds; Intraparticle convection; Pore flow; Biochemistry; Metabolism; Biokemi; metabolism; Biotechnology; Bioteknik;

   Sammanfattning : Agarose, the most commonly employed chromatography base material in industrial purification processes for biomolecules – proteins, has almost ideal properties. The major disadvantage of the agarose support is its poor mechanical strength which excludes its use in high speed separations where small particles are needed in order to achieve improved chromatographic efficiency. LÄS MER