Sökning: "TRITON X-100"

Visar resultat 1 - 5 av 13 avhandlingar innehållade orden TRITON X-100.

  1. 1. Transplantation of normal and decellularized syngeneic, allogeneic and xenogeneic cardiac tissue in mice and non-human primates

    Författare :Ketaki Methe; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Decellularization; Cardiac tissue; Syngeneic; Allogeneic; Xenogeneic; Non-human primate;

    Sammanfattning : Introduction Because of the restricted inherent capacity of regeneration and healing, transplantation is the only treatment for end-stage heart failure but it has limitations of donor shortage and graft rejection. Cardiac tissue engineering strategies might help to alleviate this problem. LÄS MER

  3. 2. Prion-infection and Cellular Signaling : Influence of scrapie-infection on lipid raft-associated proteins

    Författare :Hanna Gyllberg; Katarina Bedecs; Albert Taraboulos; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Prion diseases are a group of fatal neurodegenerative diseases affecting almost all mammals including humans. The diseases are caused by formation of the misfolded isoform of the cellular prion protein (PrPC) to the disease causing PrPSc. LÄS MER

  4. 3. Isolation of caveolae using affinity two-phase partitioning

    Författare :Parisa Abedinpour; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metabolism; Biokemi; Metabolism; Biochemistry; basolateral; apical; immunoaffinity; wheat germ agglutinin; caveolin; caveolae caveolae enriched fraction; plasma membranes; rat lung; rat liver; Affinity Partitioning; two-phase partitioning;

    Sammanfattning : The intention of this work was to establish alternative purification methods to obtain highly purified caveolae from various tissues. In order to isolate caveolae, sufficiently pure plasma membranes are needed. LÄS MER

  5. 4. mPGES-1 : a key regulator of fever and neonatal respiratory depression

    Författare :Sipra Saha; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Prostaglandins are potent lipid mediators, synthesized de novo from arachidonic acid (AA) upon cell activation. AA is oxidized by the cyclooxygenase isoenzymes (COX-1 and COX-2) to form PGH2, the common substrate for downstream enzymes involved in prostaglandin biosynthesis. LÄS MER

  7. 5. Adsorption of proteins on zeolite FAU

    Författare :Daniel Klint; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; matrix preparation; cryo-electron microscopy; protein purification; zeolites; Inorganic chemistry; Oorganisk kemi;

    Sammanfattning : A highly dealuminated form of zeolite FAU, ultra-stable zeolite Y (USY), was used as an adsorbent matrix in the purification of proteins. Undesired proteins were removed from a crude preparation by adsorption on USY, increasing the purity of an unadsorbed protein (horse radish peroxidase), or the protein of interest was adsorbed on the zeolite and subsequently eluted through changes in the solution conditions (hen egg lysozyme, mouse-immunoglobulin G). LÄS MER