Sökning: "TIRF"

Visar resultat 6 - 10 av 20 avhandlingar innehållade ordet TIRF.

  1. 6. Assay development for studies of G protein-coupled receptors at the single-molecule level

    Författare :Olov Wahlsten; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TIRF microscopy; colocalization; drug discovery; QCM-D; GPCR; pharmacodynamics; kinetics; equilibrium fluctuation analysis;

    Sammanfattning : G protein-coupled receptors (GPCRs), also known as seven transmembrane (7TM) receptors,is the largest family of cell surface receptors. These receptors play a key role in transmittinga wide variety of signals across the cell membrane and are involved in physiologicalprocesses such as sensory transduction, cell-cell communication, neuronal transmission,and hormonal signaling. LÄS MER

  3. 7. Development of novel bioanalytical assays with single-molecule readout for biomarker detection and drug candidate characterization

    Författare :Olov Wahlsten; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; QCM-D; biomarker detection; TIRF microscopy.; Bioanalytical assay; spectrofluorometry; fluorescence; single-molecule readout; drug discovery; liposomes;

    Sammanfattning : Bioanalytical assays with single-molecule readout for studying molecular interactions have in the past decades received increasing attention. The high sensitivity often offered by this readout scheme has for example enabled ultra-sensitive analyte detection, having important implications for monitoring early disease progression and the effects of drug treatment. LÄS MER

  4. 8. Binding Kinetics of Proteins at Immune-Cell Contacts

    Författare :Manto Chouliara; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; T cells; Binding Kinetics; Affinity; Lifetime; CD4; CD2; TIRF Microscopy; SLBs;

    Sammanfattning : Protein-protein interactions are crucial in numerous cellular functions and biological processes that take place inside our body. It is therefore not surprising that these interactions also govern the response of our body´s defence mechanism, the so-called immune system, towards an infection. LÄS MER

  5. 9. The influence of adhesion molecules on binding and protein organization in cell contacts

    Författare :Tommy Dam; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; T cell; SLBs; Calcium Signaling; TIRF microscopy; Two-Dimensional affinity; Protein Segregation; Protein Organization; Protein Height;

    Sammanfattning : Interactions between immune cells such as T cells and antigen-presenting cells (APCs) are integral for mounting an adaptive immune response. The interaction between the T cell receptor (TCR) and the antigen-presenting major histocompatibility complex (pMHC) on a contacting T cell and APC, is widely accepted to be the key interaction. LÄS MER

  7. 10. Towards the Characterization of Biological Nanoparticles

    Författare :Silver Jõemetsa; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; supported lipid bilayer; diffusion; size determination; microfluidics; lipophilic dyes; TIRF microscopy;

    Sammanfattning : .... LÄS MER